ART1 Protein, Cynomolgus (HEK293, His)

ART1 Protein is an arginine-specific ADP-ribosyltransferase. ART1 is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway. ART1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ART1 protein, expressed by HEK293 , with C-His labeled tag. The total length of ART1 Protein, Cynomolgus (HEK293, His) is 272 a.a., with molecular weight of 33-43 KDa.

NAD(P)(+)--arginine ADP-ribosyltransferase (ART1), an arginine-specific ADP-ribosyltransferase, catalyzes the transfer of ADP-ribose moiety to arginine residues on an acceptor protein using nicotinamide adenine dinucleotide (NAD+) as a substrate, thus eliciting changes in the activities and functions of the acceptor proteins. ART1 is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway. In addition, ART1 plays a role in glycolysis and energy metabolism under high glucose conditions and promotes angiogenesis in colorectal cancer cells through the PI3K/AKT pathway^[1].

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 17.43 ng/mL. Corresponding to a specific activity is 5.737×10^4 Unit/mg.

Cynomolgus

HEK293

C-6*His

XP_005579037 (Q23-N294)

102142531  [NCBI]

QSHPITRRDLFSQEMPLDMALASFDDQYAGCAAAMTAALPDLNHTEFQANKVYADGWTLASSQWQERQAWGPEWSLSPTRPPPPPLGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLGRGQRPPRCHQVFRGVHGLHFRPAGPGATVRLGGFASASLKNVAAQQFGEDTFFGIWTCLGAPIKGYSFFPGEEEVLIPPFETFQVINASRPAQGPARIYLRALGKRSTYNCEYIKDKKCKSGPCHLDN

Approximately 33-34 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.