B3GAT1 Protein, Human (HEK293, His)

B3GAT1 Protein is pivotal in L2/HNK-1 carbohydrate epitope biosynthesis on glycoproteins and glycosaminoglycan biosynthesis. Catalyzing substrates like ASOR and asialo-fetuin, its enzymatic function depends on sphingomyelin presence, with stearoyl-sphingomyelin being the most effective. Notably, it exclusively acts on saturated fatty acids in sphingomyelin, showing no reactivity with unsaturated fatty acids, regardless of acyl group length. B3GAT1 Protein, Human (HEK293, His) is the recombinant human-derived B3GAT1 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of B3GAT1 Protein, Human (HEK293, His) is 310 a.a., with molecular weight of 50-60 kDa.

The B3GAT1 Protein plays a key role in the biosynthesis of the L2/HNK-1 carbohydrate epitope on glycoproteins and is implicated in glycosaminoglycan biosynthesis. The substrates involved in its catalytic activity encompass asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Intriguingly, the protein's enzymatic function is contingent upon the presence of sphingomyelin, with stearoyl-sphingomyelin proving to be the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Notably, the protein demonstrates activity exclusively with sphingomyelin harboring a saturated fatty acid, showing no reactivity with unsaturated fatty acids, irrespective of acyl group length.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

N-6*His

Q9P2W7 (H25-I334)

27087  [NCBI]

HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

50-60 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.