1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Erythrocyte CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. CD239/BCAM Immunoglobulin-like Cell Adhesion Molecules
  5. Basal Cell Adhesion Molecule (BCAM)
  6. BCAM Protein, Rat (HEK293, His)

BCAM Protein, Rat (HEK293, His)

Cat. No.: HY-P72845
COA Handling Instructions

Nectin-2/CD112 Protein, functioning as a laminin alpha-5 receptor, displays potential involvement in intracellular signaling, reflecting its similarity to related proteins. BCAM Protein, Rat (HEK293, His) is the recombinant rat-derived BCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of BCAM Protein, Rat (HEK293, His) is 543 a.a., with molecular weight of ~68 kDa.

For research use only. We do not sell to patients.

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Description

Nectin-2/CD112 Protein, functioning as a laminin alpha-5 receptor, displays potential involvement in intracellular signaling, reflecting its similarity to related proteins. BCAM Protein, Rat (HEK293, His) is the recombinant rat-derived BCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of BCAM Protein, Rat (HEK293, His) is 543 a.a., with molecular weight of ~68 kDa.

Background

The Nectin-2/CD112 protein serves as a laminin alpha-5 receptor and exhibits potential for mediating intracellular signaling, as inferred by its similarity to related proteins.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells.When cells are added to coated plates(10 μg/mL,100 μL/well), approximately 20-50% will adhere after 1 hour at 37℃.

Species

Rat

Source

HEK293

Tag

C-His

Accession

Q9ESS6 (M1-A543)

Gene ID

78958  [NCBI]

Molecular Construction
N-term
BCAM (M1-A543)
Accession # Q9ESS6
His
C-term
Synonyms
Basal cell adhesion molecule; B-CAM cell surface glycoprotein; CD239; Bcam; Gplu; Lu
AA Sequence

MEPPDARAGLLWLTLLLSGYSGAQAELHVSVPPRVEVMRGEQIALDCTPREHPENYVLEWLLVDASGARHRLASVEPQGSEFLGTIHNSRGRRPPYKIDSLGRLVIAEAQVGDERDYVCVVKAGAAGTSEATSSVRVFATPEATEVAPNKGTLSVMEQFAQEIATCSSNNGNPVPRITWYQNGQRLDVPMELNSKGYMTSRTVREASGLYSLTSTLYLRLHKEDRDASFHCAAHYDLPSGQHGRLDSHTFRLTLHYPTEHVEFWVGSPSTTEGWVREGDAVQLLCQGDGSPSPEYSFFREQGNQEEQLNVNLKGNLTLEGVHRSQSGIYGCRVEDYDADEEVQLVKKLKLHVAYLDPLELSVPEEFSVFLNSSGTVVNCSARGLPAPIVRWTKDSVTVADGPILSLDSVTFDSAGTYTCEASTPTVPLLSRTQSFQLVVQGAPELKPNEIKPKSGTSWTEGDEVMLTCSARGFPEPKLTWSQRGDTTPAEPPFEGRGWMSSSLTLKVTSALSREGVSCEASNIHGKNGHVFHFGSVAPQTAQA

Molecular Weight

Approximately 68 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BCAM Protein, Rat (HEK293, His)
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HY-P72845
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