2. Recombinant Proteins
  3. CD Antigens Receptor Proteins
  4. Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Epithelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  5. CD324/E-Cadherin Cadherins
  6. E-Cadherin/Cadherin-1
  7. Cadherin-1/CD324 Protein, Human (HEK293, C-His)

Cadherin-1/CD324 Protein, Human (HEK293, C-His)

Cat. No.: HY-P7697A
Handling Instructions

Cadherin-1/CD324 Protein, Human (HEK293, His) is a recombinant human E-cadherin produced in HEK293 cells, with His tag. Cadherin-1/CD324/E-cadherin is one of the most important molecules in cell-cell adhesion in epithelial tissues.

For research use only. We do not sell to patients.

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This product has been "discontinued". Optimized version of product available: HY-P7697

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Description

Cadherin-1/CD324 Protein, Human (HEK293, His) is a recombinant human E-cadherin produced in HEK293 cells, with His tag. Cadherin-1/CD324/E-cadherin is one of the most important molecules in cell-cell adhesion in epithelial tissues[1].
Background

Cadherin-1/CD324/E-cadherin/CAM 120/80 is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. As a member of a large family of genes coding for calcium-dependent cell adhesion molecules (CAMs), the cadherin glycoproteins are expressed by a variety of tissues, mediating adhesion through homotypic binding. Classical cadherins-E- and N-cadherins being the best characterized-play important roles in the formation of tissues during gastrulation, neurulation and organogenesis[1].
Species

Human
Source

HEK293
Tag

C-6*His
Accession

P12830 (D155-I707)
Gene ID

999  [NCBI]
Molecular Construction
N-term
CADH1 (D155-I707)
Accession # P12830
6*His
C-term
Synonyms
rHuCadherin-1/CD324, His; Cadherin-1; CDH1; CAM 120/80; E-cadherin; CD324
AA Sequence

DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIHHHHHH

Molecular Weight

Approximately 75.59 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Cadherin-1/CD324 Protein, Human (HEK293, C-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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