Cadherin-13 Protein, Mouse (HEK293, His)

Cadherin-13 proteins are calcium-dependent cell adhesion molecules that exhibit a preference for homogeneous interactions, promote connections between adjacent cells, and may play a role in cell type sorting. Cadherin-13 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Cadherin-13 protein, expressed by HEK293 , with C-His labeled tag. The total length of Cadherin-13 Protein, Mouse (HEK293, His) is 692 a.a., with molecular weight of 90-110 kDa.

Cadherin-13, a calcium-dependent cell adhesion protein, stands out for its unique features in comparison to classical cadherins. Unlike the typical cadherin EC1 domain strand-swapping mechanism for homodimerization, Cadherin-13 achieves this through a distinct homophilic adhesive interface, connecting two elongated EC1-EC2 domains near their Ca2+-binding sites. This arrangement forms a tetrahedral, X-like shape, showcasing a different mode of homodimerization. This protein is implicated in potentially acting as a negative regulator of neural cell growth, and its ability to preferentially interact with itself in a homophilic manner suggests a role in contributing to the sorting of heterogeneous cell types within neural tissues.

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. When 5 x 10⁴ cells/well are added to Recombinant Mouse Cadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED₅₀ for this effect is 4.025 μg/mL, corresponding to a specific activity is 248.447 units/mg.

Mouse

HEK293

C-10*His

Q9WTR5 (M1-A692)

12554  [NCBI]

DDLECTPGFQRKVLHIHQPAEFIEDQPVLNLTFNDCKGNEKLHYEVSSPHFKVNSDGTLVALRNITAVGRTLFVHARTPHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPEGSKFRLTGKGVDQDPKGTFRINENTGSVSVTRTLDRETIATYQLYVETTDASGKTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIVIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPTTGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTKQENISVGSVLLTVNATDPDSLQHQTIRYSIYKDPAGWLSINPINGTVDTTAVLDRESPFVHNSVYTALFLAIDSGNPPATGTGTLLITLEDINDNAPVIYPTVAEVCDDARNLSVVILGASDKDLHPNTDPFKFEIHKQTVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCKNSKVDCNGA

Approximately 90-110 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.