CD300c Protein, Human (183a.a, HEK293, His)

CD300c protein, also known as CMRF35 antigen, was identified using a monoclonal antibody. It is present on immune cells like monocytes, neutrophils, and certain T and B lymphocytes, as documented by Jackson et al. in 1992. The protein is broadly expressed in various tissues, including the lung, appendix, and 19 other tissues. CD300c Protein, Human (183a.a, HEK293, His) is the recombinant human-derived CD300c protein, expressed by HEK293 , with C-His labeled tag. The total length of CD300c Protein, Human (183a.a, HEK293, His) is 155 a.a., with molecular weight of 29-40 kDa.

The CD300c protein, also known as the CMRF35 antigen, has been identified through reactivity with a monoclonal antibody. This antigen is found on various immune cells, including monocytes, neutrophils, and certain T and B lymphocytes, as documented in a study by Jackson et al. in 1992. The protein exhibits broad expression across multiple tissues, with notable presence in lung, appendix, and 19 other tissues.

Measured by its ability to inhibit anti-CD3-induced proliferation of Jurkat human T-lymphocyte leukemia cells. The ED₅₀ this effect is 3.884 μg/ml, corresponding to a specific activity is 257.47 units/mg.

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  Measured by its ability to inhibit anti-CD3-induced proliferation of Jurkat human T-lymphocyte leukemia cells. The ED₅₀ this effect is 3.884 μg/mL, corresponding to a specific activity is 257.47 units/mg.

Human

HEK293

C-His

NP_006669.1 (M29-R183)

10871  [NCBI]

29-40 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.