CD36 Protein, Rat (HEK293, His)

CD36, a versatile glycoprotein, acts as a receptor for various ligands, including thrombospondin, collagen, and oxidized LDL. Its multivalent ligands induce CD36 clusters, initiating signal transduction. CD36's roles span angiogenesis, inflammation, fatty acid metabolism, and taste perception. It facilitates fatty acid transport, influencing muscle utilization, adipose storage, and gut absorption. Mechanistically, it activates LYN kinase, leading to CD36 depalmitoylation and endocytosis. In the small intestine, CD36 aids dietary absorption through MAPK1/3 signaling. It also regulates oral fat perception, hypothalamic function, and contributes to antiangiogenic and inflammatory effects. Additionally, CD36 selectively senses microbial lipopeptides, triggering cytokine production via TLR2:TLR6 heterodimer signaling. CD36 Protein, Rat (HEK293, His) is the recombinant rat-derived CD36 protein, expressed by HEK293, with C-His labeled tag. The total length of CD36 Protein, Rat (HEK293, His) is 410 a.a., with molecular weight of 70-80 kDa.

CD36 is a multifunctional glycoprotein serving as a receptor for a diverse array of ligands encompassing proteinaceous entities like thrombospondin, fibronectin, collagen, or amyloid-beta, as well as lipidic components including oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids, and bacterial diacylated lipopeptides. Due to their multivalent nature, these ligands can engage multiple receptors concurrently, prompting the formation of CD36 clusters that initiate signal transduction and the internalization of receptor-ligand complexes. The ensuing cellular responses play pivotal roles in angiogenesis, inflammatory responses, fatty acid metabolism, and the processing of taste and dietary fats in the intestine. CD36's involvement in binding long-chain fatty acids facilitates their transport into cells, contributing to muscle lipid utilization, adipose energy storage, and gut fat absorption. Mechanistically, fatty acid binding activates downstream kinase LYN, leading to the phosphorylation and inactivation of palmitoyltransferase ZDHHC5, subsequently resulting in CD36 depalmitoylation and caveolar endocytosis. In the small intestine, CD36 participates in the proximal absorption of dietary fatty acids and cholesterol, potentially through the activation of the MAPK1/3 (ERK1/2) signaling pathway. Furthermore, CD36 is crucial for oral fat perception and preferences, with its presence in taste receptor cells mediating intracellular calcium level increases and activating gustatory neurons. In the ventromedial hypothalamus, CD36 plays a pivotal role in sensing long-chain fatty acids and regulating energy and glucose homeostasis. Acting as a receptor for thrombospondins and as a coreceptor for TLR4:TLR6 heterodimer, CD36 promotes antiangiogenic effects and inflammation in monocytes/macrophages, respectively. Upon ligand binding, CD36 interacts with the TLR4:TLR6 heterodimer, leading to internalization and triggering inflammatory responses via NF-kappa-B-dependent pathways. Additionally, CD36 serves as a selective and nonredundant sensor of microbial diacylated lipopeptides, eliciting NF-kappa-B-dependent cytokine production and subsequent Golgi targeting in a lipid-raft dependent pathway through TLR2:TLR6 heterodimer signaling.

Immobilized Rat CD36, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC₅₀ of 7.9 ng/mL determined by ELISA.

Rat

HEK293

C-His

Q07969 (G30-K439)

29184  [NCBI]

GDLLIEKTIKREVVLEEGTIAFKNWVKTGTTVYRQFWIFDVQNPEEVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPKDSTVSFVQPNGAIFEPSLSVGTENDNFTVLNLAVAAAPHIYTNSFVQGVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNNTVDGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESYCDMINGTDAASFPPFVEKSQTLRFFSSDICRSIYAVFESEVNLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLNPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFRNQVTGKIK

70-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.