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  4. COL21A1 Protein, Human (HEK293, His)

COL21A1 Protein, Human (HEK293, His)

Cat. No.: HY-P70094
Handling Instructions

COL21A1 Protein is a member of the fibre-associated collagen interrupt helix (FACIT) family. COL21A1 Protein is a secreted protein widely present in a variety of tissues. COL21A1 Protein may contribute to the extracellular matrix assembly of vascular networks during angiogenesis. COL21A1 Protein, Human (HEK293, His) is the recombinant human-derived COL21A1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of COL21A1 Protein, Human (HEK293, His) is 935 a.a., with molecular weight of 115-125 & 220 kDa, respectively.

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Description

COL21A1 Protein is a member of the fibre-associated collagen interrupt helix (FACIT) family. COL21A1 Protein is a secreted protein widely present in a variety of tissues. COL21A1 Protein may contribute to the extracellular matrix assembly of vascular networks during angiogenesis. COL21A1 Protein, Human (HEK293, His) is the recombinant human-derived COL21A1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of COL21A1 Protein, Human (HEK293, His) is 935 a.a., with molecular weight of 115-125 & 220 kDa, respectively.
Background

Collagen alpha-1 (XXI) chain is a protein encoded in humans by the COL21A1 gene and is a member of the FACIT (Fibrous Associated Collagen Interrupt Helix) collagen family. The expression of COL21A1 in human tissues is regulated by development, and the expression level is higher in the fetal stage. Type XXI collagen is an extracellular matrix component of blood vessel walls and is secreted by smooth muscle cells. Platelet-derived growth factor (PDGF) has a significant effect on the stimulation of COL21A1 expression in cultured aortic smooth muscle cells, suggesting that alpha1 (XXI) collagen may contribute to the extracellular matrix assembly of vascular networks during angiogenesis[1][2][3][4][5].
Species

Human
Source

HEK293
Tag

C-6*His
Accession

Q96P44 (E23-Y957)
Gene ID
Molecular Construction
N-term
COL21A1 (E23-Y957)
Accession # Q96P44
6*His
C-term
Synonyms
rHuCollagen alpha-1(XXI) chain/COL21A1, His ; COL21A1; collagen, type XXI, alpha 1; collagen alpha-1(XXI) chain; alpha 1 type XXI collagen; FP633; COLA1L; dJ708F5.1; dJ682J15.1; FLJ39125; FLJ44623; MGC26619; DKFZp564B052
AA Sequence

EDGEVRSSCRTAPTDLVFILDGSYSVGPENFEIVKKWLVNITKNFDIGPKFIQVGVVQYSDYPVLEIPLGSYDSGEHLTAAVESILYLGGNTKTGKAIQFALDYLFAKSSRFLTKIAVVLTDGKSQDDVKDAAQAARDSKITLFAIGVGSETEDAELRAIANKPSSTYVFYVEDYIAISKIREVMKQKLCEESVCPTRIPVAARDERGFDILLGLDVNKKVKKRIQLSPKKIKGYEVTSKVDLSELTSNVFPEGLPPSYVFVSTQRFKVKKIWDLWRILTIDGRPQIAVTLNGVDKILLFTTTSVINGSQVVTFANPQVKTLFDEGWHQIRLLVTEQDVTLYIDDQQIENKPLHPVLGILINGQTQIGKYSGKEETVQFDVQKLRIYCDPEQNNRETACEIPGFNGECLNGPSDVGSTPAPCICPPGKPGLQGPKGDPGLPGNPGYPGQPGQDGKPGYQGIAGTPGVPGSPGIQGARGLPGYKGEPGRDGDKGDRGLPGFPGLHGMPGSKGEMGAKGDKGSPGFYGKKGAKGEKGNAGFPGLPGPAGEPGRHGKDGLMGSPGFKGEAGSPGAPGQDGTRGEPGIPGFPGNRGLMGQKGEIGPPGQQGKKGAPGMPGLMGSNGSPGQPGTPGSKGSKGEPGIQGMPGASGLKGEPGATGSPGEPGYMGLPGIQGKKGDKGNQGEKGIQGQKGENGRQGIPGQQGIQGHHGAKGERGEKGEPGVRGAIGSKGESGVDGLMGPAGPKGQPGDPGPQGPPGLDGKPGREFSEQFIRQVCTDVIRAQLPVLLQSGRIRNCDHCLSQHGSPGIPGPPGPIGPEGPRGLPGLPGRDGVPGLVGVPGRPGVRGLKGLPGRNGEKGSQGFGYPGEQGPPGPPGPEGPPGISKEGPPGDPGLPGKDGDHGKPGIQGQPGPPGICDPSLCFSVIARRDPFRKGPNY

Molecular Weight

115-125&220 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

COL21A1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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