Elastase IIIB/CELA3B Protein, Mouse (Myc, His)

The elastase IIIB/CELA3B protein exhibits remarkable efficiency as a protease, showing a clear preference for alanine specificity while exhibiting limited elastolytic activity. This unique zymogram makes this protein a promising candidate for precise substrate cleavage, especially when processing alanine-rich substrates. Elastase IIIB/CELA3B Protein, Mouse (Myc, His) is the recombinant mouse-derived Elastase IIIB/CELA3B protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Elastase IIIB/CELA3B Protein, Mouse (Myc, His) is 242 a.a., with molecular weight of ~31.1 kDa.

Elastase IIIB, also known as CELA3B, is an efficient protease with a notable specificity for alanine but exhibits only limited elastolytic activity. This enzyme is characterized by its ability to cleave peptide bonds specifically at alanine residues, indicating a preference for substrates containing this amino acid. While it does not demonstrate strong elastolytic activity, its efficient proteolytic function suggests a role in the targeted cleavage of proteins containing alanine residues. The unique substrate specificity of Elastase IIIB/CELA3B underscores its distinctive role in proteolytic processes within biological systems.

Mouse

E. coli

N-His;C-Myc

Q9CQ52 (28V-269N)

67868  [NCBI]

VVNGEEAVPHSWPWQVSLQYEKDGSFHHTCGGSLITPDWVLTAGHCISTSRTYQVVLGEHERGVEEGQEQVIPINAGDLFVHPKWNSMCVSCGNDIALVKLSRSAQLGDAVQLACLPPAGEILPNGAPCYISGWGRLSTNGPLPDKLQQALLPVVDYEHCSRWNWWGLSVKTTMVCAGGDIQSGCNGDSGGPLNCPADNGTWQVHGVTSFVSSLGCNTLRKPTVFTRVSAFIDWIEETIANN

Approximately 31.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.