EphB4 Protein, Human (HEK293, His)

EphB4 protein is a receptor tyrosine kinase that binds to ephrin B ligand and initiates bidirectional signaling. Positive signaling regulates the rejection and separation of cells from EFNB2-expressing cells. EphB4 Protein, Human (HEK293, His) is the recombinant human-derived EphB4 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB4 Protein, Human (HEK293, His) is 524 a.a., with molecular weight of 60-70 kDa.

EphB4 protein, a receptor tyrosine kinase, engages in promiscuous binding to transmembrane ephrin-B family ligands located on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is termed forward signaling, while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Collaborating with its cognate ligand, EFNB2, EphB4 is intricately involved in regulating cell adhesion and migration. Moreover, EphB4 assumes a central role in heart morphogenesis, angiogenesis, and the remodeling and permeability of blood vessels. The forward signaling mediated by EPHB4 is instrumental in controlling cellular repulsion and segregation from EFNB2-expressing cells.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-His

P54760 (L16-A539)

2050  [NCBI]

60-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.