FGF-21 Protein, Hamster (HEK293, His)

FGF21 is a liver factor that signals through the FGF21 receptor in the paraventricular nucleus of the hypothalamus to regulate monosaccharide intake and preference for sweets. FGF21 promotes the progression of non-small cell lung cancer by activating the SIRT1/PI3K/AKT signaling pathway. FGF21 plays an important role in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 Protein, Hamster (HEK293, His) is the recombinant FGF-21 protein, expressed by HEK293 , with C-His labeled tag. The total length of FGF-21 Protein, Hamster (HEK293, His) is 209 a.a., with molecular weight of ~21.16 kDa.

Fibroblast growth factor 21 is a member of the fibroblast growth factor (FGF) family. FGF family members have a wide range of mitotic and cell survival activities and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF21 is a hepatic factor, a hormone secreted by the liver that signals through FGF21 receptors in the paraventricular nucleus of the hypothalamus to regulate monosaccharide intake and preference for sweets and is associated with reduced dopamine neurotransmission within the nucleus accumbens. FGF21 promotes progression of non-small cell lung cancer by activating the SIRT1/PI3K/AKT signaling pathway^[1][2][3][4].

Hamster FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 264.50 nM as determined in SPR assay (Biacore T200).

Others

HEK293

C-His

XP_005084765 (M1-S209)

101842115  [NCBI]

Approximately 21.16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.