GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, His)

GFRA1/GDNFR-α-1 protein is a receptor for GDNF and critically mediates GDNF-induced autophosphorylation and RET receptor activation. Presumably, two GDNFR-α-1 molecules form a complex with disulfide-linked GDNF, two RET molecules, and SORL1, suggesting the existence of a coordinated signaling mechanism. GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, His) is the recombinant rat-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, His) is 421 a.a., with molecular weight of 58-65 kDa.

GFRA1/GDNFR-alpha-1 Protein serves as a receptor for GDNF and plays a crucial role in mediating the GDNF-induced autophosphorylation and activation of the RET receptor. It is proposed that two molecules of GDNFR-alpha-1 form a complex with the disulfide-linked GDNF dimer and two molecules of RET, suggesting a coordinated mechanism for signal transduction. GFRA1 interacts with RET, facilitating the intricate signaling pathways associated with GDNF-induced responses. Additionally, GFRA1 engages in interactions with SORL1, either alone or in a complex with GDNF. This interaction results in the internalization of GFRA1, without leading to its degradation, indicating a regulatory aspect of GFRA1 dynamics. These molecular interactions highlight the multifaceted role of GFRA1 in mediating GDNF signaling and emphasize its importance in cellular processes.

Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED₅₀ for this effect is ≤0.8977 μg/mL, corresponding to a specific activity is ≥1114 units/mg.

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  Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells.The ED₅₀ for this effect is 0.5294 μg/mL, corresponding to a specific activity is 1688.0486 units/mg.

Rat

HEK293

C-His

Q62997-1 (D25-L445)

25454  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISDVFQQVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEERERPNCLSLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYVDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDSDFGKDGLAGASSHITTKSMAAPPSCSL

Approximately 58-70 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.