Glypican-1/GPC1 Protein, Human (HEK293, His)

Glypican-1 (GPC1) is a heparan sulfate-containing cell surface proteoglycan. Glypican-1/GPC1 Protein, Human (HEK293, His) is the recombinant human-derived Glypican-1/GPC1 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of Glypican-1/GPC1 Protein, Human (HEK293, His) is 506 a.a., with molecular weight of 65-150 kDa.

Glypican-1 (GPC1) Protein is a cell surface proteoglycan characterized by its association with heparan sulfate. It binds alpha-4 (V) collagen through the heparan sulfate side chains and plays a role in Schwann cell myelination. Additionally, GPC1 may act as a catalyst in the conversion of prion protein PRPN(C) to PRNP(Sc) by associating with both forms of PRPN, targeting them to lipid rafts, and facilitating their interaction. Moreover, GPC1 is essential for proper skeletal muscle differentiation, as it sequesters FGF2 in lipid rafts, preventing its binding to receptors (FGFRs) and inhibiting FGF-mediated signaling.

Human

HEK293

N-6*His

P35052 (D24-T529)

2817  [NCBI]

DPASKSRSCGEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANRSHAELETALRDSSRVLQAMLATQLRSFDDHFQHLLNDSERTLQATFPGAFGELYTQNARAFRDLYSELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLLPDDYLDCLGKQAEALRPFGEAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLGPECSRAVMKLVYCAHCLGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNPQGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMARGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRSAYNGNDVDFQDASDDGSGSGSGDGCLDDLCSRKVSRKSSSSRTPLTHALPGLSEQEGQKT

65-150 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.