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  4. IFN-γ
  5. IFN-gamma Protein, Mouse (155a.a, HEK293)

IFN-gamma Protein, Mouse (155a.a, HEK293)

Cat. No.: HY-P73251
Handling Instructions

IFN-gamma protein, a type II interferon, is a soluble cytokine secreted by cells in the immune systems, acting as a homodimer to bind the interferon gamma receptor and trigger a cellular response against infections. Mice deficient in IFN-gamma display heightened susceptibility to various infections and an increased risk of autoimmune diseases. In the reference dataset, IFN-gamma exhibits low expression levels. IFN-gamma Protein, Mouse (155a.a, HEK293) is the recombinant mouse-derived IFN-gamma protein, expressed by HEK293 , with tag free. The total length of IFN-gamma Protein, Mouse (155a.a, HEK293) is 133 a.a., with molecular weight of ~17.6 & 21.7 kDa, respectively.

For research use only. We do not sell to patients.

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This product has been "discontinued". Optimized version of product available: HY-P70667

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Description

IFN-gamma protein, a type II interferon, is a soluble cytokine secreted by cells in the immune systems, acting as a homodimer to bind the interferon gamma receptor and trigger a cellular response against infections. Mice deficient in IFN-gamma display heightened susceptibility to various infections and an increased risk of autoimmune diseases. In the reference dataset, IFN-gamma exhibits low expression levels. IFN-gamma Protein, Mouse (155a.a, HEK293) is the recombinant mouse-derived IFN-gamma protein, expressed by HEK293 , with tag free. The total length of IFN-gamma Protein, Mouse (155a.a, HEK293) is 133 a.a., with molecular weight of ~17.6 & 21.7 kDa, respectively.

Background

IFN-gamma protein, a member of the type II interferon class, is a soluble cytokine secreted by cells of both the innate and adaptive immune systems. In its active form, it exists as a homodimer that binds to the interferon gamma receptor, initiating a cellular response against viral and microbial infections. Mice deficient in this gene exhibit heightened susceptibility to viral, bacterial, and parasitic infections, as well as an increased risk of several autoimmune diseases. In the reference dataset, IFN-gamma demonstrates low expression levels.

Species

Mouse

Source

HEK293

Tag

Tag Free

Accession

NP_032363.1 (H23-C155)

Gene ID

15978  [NCBI]

Molecular Construction
N-term
IFN-gamma (H23-C155)
Accession # NP_032363.1
C-term
Synonyms
IFG; IFI; IFNG; IFN-gamma; Immune interferon; Interferon gamma
AA Sequence

HGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRC

Molecular Weight

Approximately 17.6&21.7 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

IFN-gamma Protein, Mouse (155a.a, HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-gamma Protein, Mouse (155a.a, HEK293)
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