LRP-5 Protein, Human (HEK293, mFc)

LRP-5 Protein functions as a coreceptor, collaborating with frizzled receptors to initiate the canonical Wnt signaling pathway. It crucially regulates cell fate determination, self-renewal, and plays a significant role in posterior epiblast patterning during gastrulation and bone development by influencing osteoblast proliferation and differentiation. LRP-5 facilitates the formation of a signaling complex, stabilizing beta-catenin and activating TCF/LEF-mediated transcription. As a coreceptor for non-Wnt proteins like norrin/NDP, LRP-5 contributes to retinal vascular development. Interactions with partners, including FZD8, AXIN1, DKK1, MESD, KREMEN2, CSNK1E, SOST, APCDD1, and CAPRIN2, underscore its intricate involvement in diverse cellular processes. LRP-5 Protein, Human (HEK293, mFc) is the recombinant human-derived LRP-5 protein, expressed by HEK293, with C-mFc labeled tag. The total length of LRP-5 Protein, Human (HEK293, mFc) is 620 a.a., with molecular weight of 110-115 kDa.

LRP-5 protein acts as a pivotal coreceptor, partnering with the frizzled family of seven-transmembrane spanning receptors to transduce signals initiated by Wnt proteins. This activation leads to the canonical Wnt signaling pathway, a critical regulator of cell fate determination and self-renewal during both embryonic development and adult tissue regeneration. LRP-5 is particularly implicated in the posterior patterning of the epiblast during gastrulation and plays a crucial role in bone development by regulating osteoblast proliferation and differentiation. Mechanistically, LRP-5 facilitates the formation of the signaling complex between Wnt ligands, frizzled receptors, and itself, leading to the recruitment of AXIN1. This stabilizes beta-catenin/CTNNB1 and activates TCF/LEF-mediated transcriptional programs. Moreover, LRP-5 serves as a coreceptor for non-Wnt proteins, such as norrin/NDP, with a demonstrated role in retinal vascular development. The interaction with various partners, including FZD8, AXIN1, DKK1, MESD, KREMEN2, CSNK1E, SOST, APCDD1, and CAPRIN2, highlights its intricate involvement in the regulation of diverse cellular processes.

Immobilized Human DKK1, His Tag at 5 μg/mL (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC₅₀ of 0.11 μg/mL determined by ELISA.

Human

HEK293

C-mFc

O75197 (E644-Q1263)

4041  [NCBI]

110-115 kDa

Greater than 90% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.