MAG/Siglec-4a Protein, Mouse (HEK293, His, solution)

The MAG/Siglec-4a protein mediates myelinating cell-neuron interactions by binding to gangliosides, RTN4R, and RTN4RL2. It maintains normal axon myelination and protects motoneurons from apoptosis after injury. It prevents degeneration of myelinated axons in adults by binding to gangliosides. Moreover, it inhibits neurite outgrowth by interacting with RTN4R, RTN4RL2, and gangliosides. It can exist as a monomer or homodimer and interacts with BSG isoform 2. MAG/Siglec-4a Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived MAG/Siglec-4a protein, expressed by HEK293, with C-6*His labeled tag. The total length of MAG/Siglec-4a Protein, Mouse (HEK293, His, solution) is 497 a.a., with molecular weight of 70-120 kDa.

The MAG/Siglec-4a protein is an adhesion molecule that plays a crucial role in mediating interactions between myelinating cells and neurons. It achieves this by binding to neuronal gangliosides containing sialic acid and to the glycoproteins RTN4R and RTN4RL2. While it is not necessary for initial myelination, it is involved in maintaining normal axon myelination. Additionally, it offers protection against apoptosis in motoneurons, particularly after injury, likely through its interaction with neuronal RTN4R and RTN4RL2. In adults, it is essential for preventing the degeneration of myelinated axons, and this process is likely dependent on its binding to gangliosides on the axon cell membrane. Moreover, the MAG/Siglec-4a protein acts as a negative regulator of neurite outgrowth by inhibiting axon growth longitudinally. Its inhibitory effects on neurite extension are mediated primarily through its interaction with neuronal RTN4R, RTN4RL2, and gangliosides. It binds preferentially to alpha-2,3-linked sialic acid and ganglioside Gt1b. The MAG/Siglec-4a protein can exist as a monomer or homodimer and interacts with isoform 2 of BSG through its first three N-terminal Ig-like domains.

Mouse

HEK293

C-6*His

P20917 (G20-P516)

17136  [NCBI]

GHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGEDGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

70-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.