ST2/IL1RL1 Protein, Mouse (HEK293, His)

The ST2/IL1RL1 protein serves as a receptor for IL-33 and plays a crucial role in immunity and response to environmental stress through IL1RAP. It recruits MYD88, IRAK1/4 and TRAF6 upon stimulation, leading to MAPK phosphorylation. ST2/IL1RL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ST2/IL1RL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ST2/IL1RL1 Protein, Mouse (HEK293, His) is 311 a.a., with molecular weight of 50-70 kDa.

ST2/IL1RL1 serves as a receptor for interleukin-33 (IL-33), playing crucial roles in innate and adaptive immunity to maintain tissue homeostasis and respond to environmental stresses, in conjunction with its coreceptor IL1RAP. Upon stimulation, it recruits MYD88, IRAK1, IRAK4, and TRAF6, leading to the phosphorylation of various MAPKs, including MAPK3/ERK1, MAPK1/ERK2, MAPK14, and MAPK8. This receptor is implicated in helper T-cell function and, in the context of tissue injury, induces a UCP2-dependent mitochondrial rewiring. This rewiring attenuates the generation of reactive oxygen species, preserving the integrity of the Krebs cycle. This preservation is essential for sustained production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. Additionally, ST2/IL1RL1 plays a role in inhibiting IL-33 signaling.

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 34.04 ng/mL in the presence of 20 ng/mL recombinant mouse IL-33, corresponding to a specific activity is 2.51×10⁵ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 34.04 ng/mL in the presence of 20 ng/mL recombinant mouse IL-33, corresponding to a specific activity is 2.51×10⁵ units/mg.

Mouse

HEK293

C-6*His

P14719-2 (S27-A337)

17082  [NCBI]

SKSSWGLENEALIVRCPQRGRSTYPVEWYYSDTNESIPTQKRNRIFVSRDRLKFLPARVEDSGIYACVIRSPNLNKTGYLNVTIHKKPPSCNIPDYLMYSTVRGSDKNFKITCPTIDLYNWTAPVQWFKNCKALQEPRFRAHRSYLFIDNVTHDDEGDYTCQFTHAENGTNYIVTATRSFTVEEKGFSMFPVITNPPYNHTMEVEIGKPASIACSACFGKGSHFLADVLWQINKTVVGNFGEARIQEEEGRNESSSNDMDCLTSVLRITGVTEKDLSLEYDCLALNLHGMIRHTIRLRRKQPSKECPSHIA

50-70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.