Tapasin Protein, Human (HEK293, His)

Tapasin protein is key to MHC class I assembly, binding to TAP and forming MHC class I with peptides. As a heterodimer of PDIA3, tapasin mediates interactions between MHC class I, calreticulin, PDIA3, and TAP. Tapasin Protein, Human (HEK293, His) is the recombinant human-derived Tapasin protein, expressed by HEK293 , with C-His labeled tag. The total length of Tapasin Protein, Human (HEK293, His) is 394 a.a., with molecular weight of 45-50 kDa.

Tapasin protein assumes a pivotal role in the intricate processes of MHC class I assembly, specifically in the association with transporter associated with antigen processing (TAP) and the formation of MHC class I with peptides during peptide loading. Forming a heterodimer with PDIA3 through disulfide linkage, tapasin becomes an obligatory mediator in the interaction among newly assembled MHC class I molecules, calreticulin, PDIA3, and TAP. Notably, up to four MHC class I/tapasin complexes bind to a single TAP, as documented in various studies. Furthermore, tapasin exhibits an additional interaction with the HLA-G-B2M complex, a critical engagement that is essential for the loading of high-affinity peptides, elucidating its multifaceted role in the orchestration of MHC class I antigen presentation.

Human

HEK293

C-6*His

O15533 (G21-V414)

6892  [NCBI]

GPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPTVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEVAGLSGPSLEDSV

Approximately 45-50 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.