UGCG Protein, Human (Cell-Free, His)

UGCG proteins play a key role in the glucosphingolipid (GSL) synthesis pathway based on glucosylceramide in the Golgi apparatus, catalyzing the key step of transferring glucose to ceramide to form glucosylceramide (GlcCer). GSLs have effects on membrane microdomains, affecting processes such as membrane trafficking and signal transduction. UGCG Protein, Human (Cell-Free, His) is the recombinant human-derived UGCG protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of UGCG Protein, Human (Cell-Free, His) is 394 a.a., with molecular weight of 47.7 kDa.

UGCG Protein serves a pivotal role in the glucosylceramide-based glycosphingolipid (GSL) synthetic pathway at the cytosolic surface of the Golgi. This enzyme catalyzes the essential initial step by transferring glucose from UDP-glucose to ceramide, yielding glucosylceramide (GlcCer), a fundamental component of GSLs. GSLs, amphipathic molecules residing in the outer leaflet of membranes, play integral roles in membrane microdomains, influencing processes like membrane trafficking and signal transduction. UGCG's contribution extends to critical cellular functions such as growth, differentiation, migration, and morphogenesis, with specific implications in nervous system development. Notably, GSLs, including GlcCer, modulate the leptin receptor/LEPR in the leptin-mediated signaling pathway and are indispensable for establishing the skin barrier, regulating keratinocyte differentiation, and assembling the cornified envelope. Furthermore, UGCG facilitates the synthesis of xylosylceramide (XylCer), showcasing its versatility in glycosphingolipid biosynthesis by utilizing UDP-Xyl as a xylose donor. This multifaceted enzymatic activity underscores UGCG's central role in diverse cellular processes and highlights its significance in maintaining cellular homeostasis and functionality.

Human

E. coli Cell-free

N-10*His

Q16739 (M1-V394)

7357

MALLDLALEGMAVFGFVLFLVLWLMHFMAIIYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPGYEVAKYDLIWICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRYYISANVTGFKCVTGMSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWRFAMSTQVAMQNSGSYSISQFQSRMIRWTKLRINMLPATIICEPISECFVASLIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQLRGVQGGTLCFSKLDYAVAWFIRESMTIYIFLSALWDPTISWRTGRYRLRCGGTAEEILDV

47.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.