2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. USP47 Protein, Human (Sf9, His, FLAG)

USP47 Protein, Human (Sf9, His, FLAG)

Cat. No.: HY-P701420
Handling Instructions

The USP47 protein is a ubiquitin-specific protease that deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizes POLB, and regulates base excision repair (BER). In addition to DNA repair, USP47 is an important regulator of cell growth and genome integrity. USP47 Protein, Human (Sf9, His, FLAG) is the recombinant human-derived USP47 protein, expressed by Sf9 insect cells , with C-Flag, N-8*His labeled tag. The total length of USP47 Protein, Human (Sf9, His, FLAG) is 1374 a.a., .

For research use only. We do not sell to patients.

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Description

The USP47 protein is a ubiquitin-specific protease that deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizes POLB, and regulates base excision repair (BER). In addition to DNA repair, USP47 is an important regulator of cell growth and genome integrity. USP47 Protein, Human (Sf9, His, FLAG) is the recombinant human-derived USP47 protein, expressed by Sf9 insect cells , with C-Flag, N-8*His labeled tag. The total length of USP47 Protein, Human (Sf9, His, FLAG) is 1374 a.a., .
Background

USP47 protein functions as a ubiquitin-specific protease with a specific role in the deubiquitination of monoubiquitinated DNA polymerase beta (POLB), thereby stabilizing POLB and contributing to the regulation of base-excision repair (BER). Beyond its role in DNA repair, USP47 emerges as a key regulator of cell growth and genome integrity. Additionally, it may exert its influence on cellular processes by indirectly modulating CDC25A expression at the transcriptional level, underscoring its multifaceted role in cellular homeostasis and genomic stability.
Species

Human
Source

Sf9 insect cells
Tag

C-Flag;N-8*His
Accession

Q96K76 (V2-D1375)
Gene ID

55031
Molecular Construction
N-term
8*His
USP47 (V2-D1375)
Accession # Q96K76
Flag
C-term
Synonyms
USP47; Ubiquitin carboxyl-terminal hydrolase 47; Deubiquitinating enzyme 47; Ubiquitin thioesterase 47; Ubiquitin-specific-processing protease 47
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

USP47 Protein, Human (Sf9, His, FLAG) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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