2. Cell Cycle/DNA Damage PI3K/Akt/mTOR Apoptosis
  3. ATM/ATR Apoptosis
  4. Mirin

Mirin is a potent Mre11-Rad50-Nbs1 (MRN) complex inhibitor. Mirin prevents MRN-dependent activation of ATM (IC50=12 μM) without affecting ATM protein kinase activity, and it inhibits Mre11-associated exonuclease activity. Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells. Mirin prevents ATM activation in response to DNA double-strand breaks (DSBs) and blocks homology-directed repair (HDR) in mammalian cells.

For research use only. We do not sell to patients.

CAS No. : 1198097-97-0

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Mirin:

Mirin Related Antibodies

Top Publications Citing Use of Products

    Mirin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Apr 3;14(1):1838.  [Abstract]

    The average euploidy rate of morula cells in the mouse IVF embryos treated with Mirin (50 μM).

    Mirin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Apr 3;14(1):1838.  [Abstract]

    Mirin (50 μM; ～16 h) significantly blocked SCNT-mediated reprogramming and retarded early embryonic development.

    Mirin purchased from MedChemExpress. Usage Cited in: Cell Rep. 2022 Nov 29;41(9):111716.  [Abstract]

    Mirin (MRE11i) (50 μM; 6 h) suppressed RPA chromatin binding and decreased H2AX phosphorylation in RPE1 BRCA1-/- TP53-/- cells compared with POLθ inhibition alone.

    Mirin purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2020 Aug 5;21(8):e48920.  [Abstract]

    Mirin (50 μM) strongly limited histone H2AX and CHK1 phosphorylation in U2OS cells to almost similar levels as maintaining the CDC7 inhibition.

    Mirin purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2020 Aug 5;21(8):e48920.  [Abstract]

    Mirin (50 μM) induced the fork restart defect in U2OS cells.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Mirin is a potent Mre11-Rad50-Nbs1 (MRN) complex inhibitor. Mirin prevents MRN-dependent activation of ATM (IC50=12 μM) without affecting ATM protein kinase activity, and it inhibits Mre11-associated exonuclease activity. Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells. Mirin prevents ATM activation in response to DNA double-strand breaks (DSBs) and blocks homology-directed repair (HDR) in mammalian cells[1][2].

    Cellular Effect
    Cell Line Type Value Description References
    Vero CC50
    > 100 μM
    Compound: 14
    Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell titer-blue fluorescent assay
    Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell titer-blue fluorescent assay
    		[PMID: 25462237]
    In Vitro

    Mirin (50, 100, 500 μM) inhibits ATM-dependent phosphorylation of Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DNA double-strand breaks (DSBs). Mirin (100 μM) inhibits Mre11 nuclease activity[1].
    Mirin (10-100 μM; 24 h) shows 50% cytotoxicity at 50 μM in HEK293 cells[1].
    Mirin (10-100 μM) induces a substantial G2 arrest at concentrations of 50 μM and 100 μM[1].
    Mirin (18 µM; 48 h) increases apoptosis in PEO4 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Mirin Related Antibodies

    Cell Cytotoxicity Assay[1]

    Cell Line: Human embryonic kidney (HEK) 293 cells
    Concentration: 10, 25, 50, 100 μM
    Incubation Time: 24 h
    Result: Had little effect on cell survival at 25 μM and showed 50% cytotoxicity at 50 μM.

    Cell Cycle Analysis[1]

    Cell Line: TOSA4 cells
    Concentration: 10, 25, 50, 100 μM
    Incubation Time:
    Result: Induced a substantial G2 arrest at concentrations of 50 μM and 100 μM.

    Apoptosis Analysis[2]

    Cell Line: PEO4 cells
    Concentration: 18 µM
    Incubation Time: 48 h
    Result: Increased apoptosis.
    Molecular Weight

    220.25

    Formula

    C10H8N2O2S
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    O=C1N=C(S/C1=C\C2=CC=C(C=C2)O)N
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 31 mg/mL (140.75 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5403 mL 22.7015 mL 45.4030 mL
    5 mM 0.9081 mL 4.5403 mL 9.0806 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1 mg/mL (4.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1 mg/mL (4.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.63%

    SDS (393 KB)

    COA (249 KB) HNMR (378 KB) RP-HPLC (245 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.5403 mL 22.7015 mL 45.4030 mL 113.5074 mL
    5 mM 0.9081 mL 4.5403 mL 9.0806 mL 22.7015 mL
    10 mM 0.4540 mL 2.2701 mL 4.5403 mL 11.3507 mL
    15 mM 0.3027 mL 1.5134 mL 3.0269 mL 7.5672 mL
    20 mM 0.2270 mL 1.1351 mL 2.2701 mL 5.6754 mL
    25 mM 0.1816 mL 0.9081 mL 1.8161 mL 4.5403 mL
    30 mM 0.1513 mL 0.7567 mL 1.5134 mL 3.7836 mL
    40 mM 0.1135 mL 0.5675 mL 1.1351 mL 2.8377 mL
    50 mM 0.0908 mL 0.4540 mL 0.9081 mL 2.2701 mL
    60 mM 0.0757 mL 0.3784 mL 0.7567 mL 1.8918 mL
    80 mM 0.0568 mL 0.2838 mL 0.5675 mL 1.4188 mL
    100 mM 0.0454 mL 0.2270 mL 0.4540 mL 1.1351 mL
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    Mirin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Mirin1198097-97-0ATM/ATRApoptosisAtaxia telangiectasia mutatedATM and RAD3 relatedMRN,Mre11Rad50Nbs1ATMexonucleaseG2/Mcheckpointhomology-directed repairHDRInhibitorinhibitorinhibit

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