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  3. BODIPY FL hydrazide

BODIPY FL Hydrazide is a green-fluorescent dye, BODIPY FL Hydrazide is reactive with aldehyde/ketone on polysaccharides and glycoproteins, yielding a reversible Schiff base product that can be transformed to a stable linkage using a reducing agent like sodium borohydride or sodium cyanoborohydride. (λex=495 nm, λem=516 nm).

For research use only. We do not sell to patients.

BODIPY FL hydrazide Chemical Structure

BODIPY FL hydrazide Chemical Structure

CAS No. : 178388-71-1

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5 mg USD 310 In-stock
10 mg USD 500 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

BODIPY FL Hydrazide is a green-fluorescent dye, BODIPY FL Hydrazide is reactive with aldehyde/ketone on polysaccharides and glycoproteins, yielding a reversible Schiff base product that can be transformed to a stable linkage using a reducing agent like sodium borohydride or sodium cyanoborohydride. (λex=495 nm, λem=516 nm)[1][2].

Molecular Weight

306.12

Formula

C14H17BF2N4O

CAS No.
Appearance

Solid

Color

Brown to red

Emission (Em)

516

Excitation (Ex)

495

SMILES

O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])NN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (408.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2667 mL 16.3335 mL 32.6669 mL
5 mM 0.6533 mL 3.2667 mL 6.5334 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2667 mL 16.3335 mL 32.6669 mL 81.6673 mL
5 mM 0.6533 mL 3.2667 mL 6.5334 mL 16.3335 mL
10 mM 0.3267 mL 1.6333 mL 3.2667 mL 8.1667 mL
15 mM 0.2178 mL 1.0889 mL 2.1778 mL 5.4445 mL
20 mM 0.1633 mL 0.8167 mL 1.6333 mL 4.0834 mL
25 mM 0.1307 mL 0.6533 mL 1.3067 mL 3.2667 mL
30 mM 0.1089 mL 0.5444 mL 1.0889 mL 2.7222 mL
40 mM 0.0817 mL 0.4083 mL 0.8167 mL 2.0417 mL
50 mM 0.0653 mL 0.3267 mL 0.6533 mL 1.6333 mL
60 mM 0.0544 mL 0.2722 mL 0.5444 mL 1.3611 mL
80 mM 0.0408 mL 0.2042 mL 0.4083 mL 1.0208 mL
100 mM 0.0327 mL 0.1633 mL 0.3267 mL 0.8167 mL
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BODIPY FL hydrazide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BODIPY FL hydrazide
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