2. Academic Validation
  3. Effect of Combination of Angiotensin-Converting Enzyme Inhibitors and Vitamin D Receptor Activators on Cardiac Oxidative Stress in Diabetic Rats

Effect of Combination of Angiotensin-Converting Enzyme Inhibitors and Vitamin D Receptor Activators on Cardiac Oxidative Stress in Diabetic Rats

  • Am J Med Sci. 2016 Aug;352(2):208-14. doi: 10.1016/j.amjms.2016.04.016.
Tarek Mohamed Ali 1,  Osama Mahmoud Mehanna 2,  Amgad Gaber Elsaid 3,  Ahmad El Askary 4
Affiliations

Affiliations

  • 1 Department of Medical Laboratory Science, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia; Department of Medical Physiology, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt. Electronic address: [email protected].
  • 2 Department of Medical Physiology, Faculty of Medicine, Taif University, Taif, Saudi Arabia; Department of Medical Physiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
  • 3 College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia; Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
  • 4 Department of Medical Laboratory Science, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia; Department of Medical Biochemistry, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
PMID: 27524220 DOI: 10.1016/j.amjms.2016.04.016
Abstract

Background: The principle mediator of diabetic myocardial injury is oxidative stress. The aim was to compare the effect of monotherapy with enalapril, angiotensin-converting Enzyme inhibitor and paricalcitol (vitamin D receptor activator), to the combined therapy with both drugs on the cardiac oxidant-antioxidant balance in the type 2 diabetic rats.

Materials and methods: A total of 50 male Sprague-Dawley rats were divided into 5 groups, namely the normal control and diabetic, vehicle, enalapril, paricalcitol and paricalcitol and enalapril-treated groups. Enalapril was given at a dose of (25mg/L) in drinking water once daily and paricalcitol was given intraperitoneally (0.8μg/kg/3 × week) for 3 months. Glycemic status, cardiac oxidant-antioxidant parameters and histologic examination were determined.

Results: Paricalcitol and combined treatment significantly (P < 0.01) reduced the level of fasting, postprandial blood glucose, homeostatic model assessment-insulin resistance, cardiac malondialdehyde and nitric oxide. Moreover, they significantly (P < 0.01) increased the levels of Insulin and c-peptide compared to diabetic control rats. Combined treatment significantly (P < 0.01) raised the level of glutathione, glutathione S-transferase and catalase more than monotherapy.

Conclusion: The combination of angiotensin-converting Enzyme inhibitors and vitamin D receptor activators has a superior effect on reducing cardiac oxidative stress by raising antioxidant activity than monotherapy in diabetic rats.

Keywords

Diabetes; Enalapril; Oxidative stress; Paricalcitol; Streptozotocin.

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