2. Academic Validation
  3. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging

Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging

  • Nat Methods. 2017 Oct;14(10):971-974. doi: 10.1038/nmeth.4400.
Hsien-Wei Yeh 1 2,  Omran Karmach 3,  Ao Ji 1,  David Carter 4,  Manuela M Martins-Green 3 4,  Hui-Wang Ai 1 2 4
Affiliations

Affiliations

  • 1 Department of Chemistry, University of California Riverside, Riverside, California, USA.
  • 2 Department of Molecular Physiology and Biological Physics and Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
  • 3 Department of Cell Biology and Neuroscience, University of California Riverside, Riverside, California, USA.
  • 4 Institute for Integrative Genome Biology, University of California Riverside, Riverside, California, USA.
PMID: 28869756 DOI: 10.1038/nmeth.4400
Abstract

Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence. One pair in particular showed superior in vitro and in vivo sensitivity over commonly used bioluminescence reporters. We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues.

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