2. Academic Validation
  3. Ultrasound-responsive low-dose doxorubicin liposomes trigger mitochondrial DNA release and activate cGAS-STING-mediated antitumour immunity

Ultrasound-responsive low-dose doxorubicin liposomes trigger mitochondrial DNA release and activate cGAS-STING-mediated antitumour immunity

  • Nat Commun. 2023 Jun 30;14(1):3877. doi: 10.1038/s41467-023-39607-x.
Chaoyu Wang 1 2,  Ruoshi Zhang 1 2,  Jia He 1 2,  Lvshan Yu 1 2,  Xinyan Li 1 2,  Junxia Zhang 2 3 4,  Sai Li 2 3 4,  Conggang Zhang 1 2,  Jonathan C Kagan 5,  Jeffrey M Karp 6 7 8 9,  Rui Kuai 10 11
Affiliations

Affiliations

  • 1 School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100084, China.
  • 2 Tsinghua-Peking Center for Life Sciences, Beijing, 100084, China.
  • 3 School of Life Sciences, Tsinghua University, Beijing, 100084, China.
  • 4 Frontier Research Center for Biological Structure & State Key Laboratory of Membrane Biology, Beijing, 100084, China.
  • 5 Division of Gastroenterology, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
  • 6 Department of Anesthesiology, Perioperative, and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • 7 Harvard-MIT Program in Health Sciences and Technology, MIT, Cambridge, MA, USA.
  • 8 Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA.
  • 9 Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • 10 School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100084, China. [email protected].
  • 11 Tsinghua-Peking Center for Life Sciences, Beijing, 100084, China. [email protected].
PMID: 37391428 DOI: 10.1038/s41467-023-39607-x
Abstract

DNA derived from chemotherapeutics-killed tumor cells is one of the most important damage-associated molecular patterns that can activate the cGAS-STING (cyclic GMP-AMP synthase-stimulator of interferon genes) pathway in antigen-presenting cells (APCs) and promote antitumor immunity. However, conventional chemotherapy displays limited tumor cell killing and ineffective transfer of stable tumor DNA to APCs. Here we show that liposomes loaded with an optimized ratio of indocyanine green and doxorubicin, denoted as LID, efficiently generate Reactive Oxygen Species upon exposure to ultrasound. LID plus ultrasound enhance the nuclear delivery of doxorubicin, induce tumor mitochondrial DNA oxidation, and promote oxidized tumor mitochondrial DNA transfer to APCs for effective activation of cGAS-STING signaling. Depleting tumor mitochondrial DNA or knocking out STING in APCs compromises the activation of APCs. Furthermore, systemic injection of LID plus ultrasound over the tumor lead to targeted cytotoxicity and STING activation, eliciting potent antitumor T cell immunity, which upon the combination with immune checkpoint blockade leads to regression of bilateral MC38, CT26, and orthotopic 4T1 tumors in female mice. Our study sheds LIGHT on the importance of oxidized tumor mitochondrial DNA in STING-mediated antitumor immunity and may inspire the development of more effective strategies for Cancer Immunotherapy.

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