Modulation of expression of epidermal Langerhans cell properties following in situ exposure to glucocorticosteroids

Langerhans cells (LC) have been implicated as antigen-presenting and target cells in contact allergic, cell-mediated reactions. We have examined the effects in guinea pigs, rats, and humans of in situ (epicutaneous) exposure to glucocorticosteroids (GCS) on the expression of epidermal LC markers. Reductions in the number of Fc-rosetting, C3b-rosetting, and immune-associated (Ia) antigen-bearing LC occurred in a dose-related fashion, with the degree of such reductions dependent upon the specific GCS employed. These reductions were determined to be reversible following cessation of exposure to GCS. T6 antigenicity, another cell surface marker of human LC, was little affected by GCS exposure. Simultaneous immunofluorescent staining for T6 and Ia antigenicity within human epidermis of amcinonide treated skin detected reduced numbers of T6+/Ia+ cells with a concomitant increase in T6+/Ia- cells. The data presented suggest a selective reduction in the expression of immunologically important receptors and antigens by LC which may be involved in steroid-responsive contact allergic reactions.