2. MAPK/ERK Pathway Immunology/Inflammation
  3. p38 MAPK NOD-like Receptor (NLR)
  4. Muramyl dipeptide

Muramyl dipeptide  (Synonyms: MDP)

Cat. No.: HY-127090 Purity: ≥98.0%
COA Handling Instructions

Molarity Calculator

Muramyl dipeptide (MDP) is a synthetic immunoreactive peptide, consisting of N-acetyl muramic acid attached to a short amino acid chain of L-Ala-D-isoGln. Muramyl dipeptide is an inducer of bone formation through induction of Runx2. Muramyl dipeptide directly enhances osteoblast differentiation by up-regulating Runx2 gene expression through MAPK pathways. Muramyl dipeptide is a NLRP1 agonist.

For research use only. We do not sell to patients.

Muramyl dipeptide Chemical Structure

Muramyl dipeptide Chemical Structure

CAS No. : 53678-77-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 242 In-stock
Solution
10 mM * 1 mL in DMSO USD 242 In-stock
Solid
1 mg USD 95 In-stock
5 mg USD 220 In-stock
10 mg USD 340 In-stock
25 mg USD 660 In-stock
50 mg USD 940 In-stock
100 mg USD 1290 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Muramyl dipeptide Related Antibodies

Top Publications Citing Use of Products

View All p38 MAPK Isoform Specific Products:

View All Isoforms

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Muramyl dipeptide (MDP) is a synthetic immunoreactive peptide, consisting of N-acetyl muramic acid attached to a short amino acid chain of L-Ala-D-isoGln. Muramyl dipeptide is an inducer of bone formation through induction of Runx2. Muramyl dipeptide directly enhances osteoblast differentiation by up-regulating Runx2 gene expression through MAPK pathways. Muramyl dipeptide is a NLRP1 agonist[1][2].

IC50 & Target[1][2]

p38 MAPK

 

NLRP1

 

In Vitro

Muramyl dipeptide (0.1-10 μg/mL; 24 hours) increases protein expression of Runx2 in a dose-dependent manner[1].
Muramyl dipeptide (0.1-10 μg/mL; 6 hours) increases mRNA levels of Runx2 in a dose-dependent manner[1].
Muramyl dipeptide indirectly attenuates osteoclast differentiation through a decreased RANKL/OPG ratio[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Muramyl dipeptide Related Antibodies

Western Blot Analysis[1]

Cell Line: MC3T3-E1 cells
Concentration: 0.1, 1, 10 µg/mL
Incubation Time: 24 hours
Result: Increased protein expression of Runx2 in a dose-dependent manner.

RT-PCR[1]

Cell Line: MC3T3-E1 cells
Concentration: 0.1, 1, 10 µg/mL
Incubation Time: 6 hours
Result: Increases mRNA levels of Runx2 in a dose-dependent manner.
In Vivo

Muramyl dipeptide (1.25 mg/kg; i.p.; twice) alleviates bone loss induced by osteoporosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: RANKL-induced osteoporosis model (Five-week-old C57BL/6 mice)[1]
Dosage: 1.25 mg/kg
Administration: i.p.; twice (RANKL-induced osteoporosis for 3 weeks and euthanized at 4 weeks)
Result: Significantly enhanced the trabecular bone volume (BV/TV) and trabecular number (Tb.N).
Clinical Trial
Molecular Weight

492.48

Formula

C19H32N4O11
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(N[C@@H](C=O)[C@H]([C@H](O)[C@H](O)CO)O[C@H](C)C(N[C@@H](C)C(N[C@@H](C(N)=O)CCC(O)=O)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (203.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (101.53 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0305 mL 10.1527 mL 20.3054 mL
5 mM 0.4061 mL 2.0305 mL 4.0611 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 5 mg/mL (10.15 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 5 mg/mL (10.15 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (248 KB) HNMR (149 KB) LCMS (287 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.0305 mL 10.1527 mL 20.3054 mL 50.7635 mL
5 mM 0.4061 mL 2.0305 mL 4.0611 mL 10.1527 mL
10 mM 0.2031 mL 1.0153 mL 2.0305 mL 5.0763 mL
15 mM 0.1354 mL 0.6768 mL 1.3537 mL 3.3842 mL
20 mM 0.1015 mL 0.5076 mL 1.0153 mL 2.5382 mL
25 mM 0.0812 mL 0.4061 mL 0.8122 mL 2.0305 mL
30 mM 0.0677 mL 0.3384 mL 0.6768 mL 1.6921 mL
40 mM 0.0508 mL 0.2538 mL 0.5076 mL 1.2691 mL
50 mM 0.0406 mL 0.2031 mL 0.4061 mL 1.0153 mL
60 mM 0.0338 mL 0.1692 mL 0.3384 mL 0.8461 mL
80 mM 0.0254 mL 0.1269 mL 0.2538 mL 0.6345 mL
100 mM 0.0203 mL 0.1015 mL 0.2031 mL 0.5076 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Muramyl dipeptide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Muramyl dipeptide53678-77-6MDPp38 MAPKNOD-like Receptor (NLR)peptidoglycansboneformationNod2osteoblastosteoclastdifferentiationRANKL/OPGratioinductionInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Muramyl dipeptide
Cat. No.:
HY-127090
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.