2. Apoptosis
  3. Caspase
  4. MX1013

MX1013  (Synonyms: CV1013; Z-VD-FMK)

Cat. No.: HY-10397A Purity: 96.32%
COA Handling Instructions

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MX1013 is a potent, irreversible dipeptide caspase inhibitor vith antiapoptotic activity. MX1013 inhibits recombinant human caspase 3 with an IC50 of 30 nM.

For research use only. We do not sell to patients.

MX1013 Chemical Structure

MX1013 Chemical Structure

CAS No. : 582316-00-5

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Based on 2 publication(s) in Google Scholar

MX1013 Related Antibodies

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Description

MX1013 is a potent, irreversible dipeptide caspase inhibitor vith antiapoptotic activity. MX1013 inhibits recombinant human caspase 3 with an IC50 of 30 nM[1].

IC50 & Target[1]

Caspase

 

In Vitro

MX1013 inhibits caspases 1, 3, 6, 7, 8, and 9, with IC50 values ranging from 5 to 20 nm. MX1013 is selective for caspases, and is a poor inhibitor of noncaspase proteases, such as cathepsin B, calpain I, or Factor Xa (IC50 values >10 μm)[1].
MX1013 inhibits three key markers of apoptosis: the proteolytic maturation of caspase 3, the caspase-mediated cleavage of PARP, and the fragmentation of genomic DNA[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MX1013 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Jurkat T-lymphocytes
Concentration: 0, 0.05, 0.1, 0.25, 0.5 μM
Incubation Time: Preincubated for 2 h
Result: Neither caspase 3 processing nor PARP cleavage could be detected at 0.5 μM. At concentrations of 0.05 μM, caspase 3 processing and PARP cleavage were still markedly reduced.
In Vivo

MX1013 is an effective antiapoptotic agent in vivo. MX1013 not only inhibits local tissue apoptosis, but also can protect animals against its lethal effects[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female ND4 Swiss Webster mice (16.5-21 g)[1]
Dosage: 0, 0.25, 1, 10 mg/kg (formulated in an aqueous vehicle containing 50 mm Tris-HCl, pH 8.0)
Administration: Injected i.v.
Result: The lowest dose of 0.25 mg/kg protected 66% of the mice from the lethal effects of anti-Fas antibody at the 3 h time point, and 1 and 10 mg/kg dose protected 100% of the mice at the 3 h time point.
Molecular Weight

382.38

Formula

C18H23FN2O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CC(NC([C@@H](NC(OCC1=CC=CC=C1)=O)C(C)C)=O)C(CF)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (261.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6152 mL 13.0760 mL 26.1520 mL
5 mM 0.5230 mL 2.6152 mL 5.2304 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

COA (246 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6152 mL 13.0760 mL 26.1520 mL 65.3800 mL
5 mM 0.5230 mL 2.6152 mL 5.2304 mL 13.0760 mL
10 mM 0.2615 mL 1.3076 mL 2.6152 mL 6.5380 mL
15 mM 0.1743 mL 0.8717 mL 1.7435 mL 4.3587 mL
20 mM 0.1308 mL 0.6538 mL 1.3076 mL 3.2690 mL
25 mM 0.1046 mL 0.5230 mL 1.0461 mL 2.6152 mL
30 mM 0.0872 mL 0.4359 mL 0.8717 mL 2.1793 mL
40 mM 0.0654 mL 0.3269 mL 0.6538 mL 1.6345 mL
50 mM 0.0523 mL 0.2615 mL 0.5230 mL 1.3076 mL
60 mM 0.0436 mL 0.2179 mL 0.4359 mL 1.0897 mL
80 mM 0.0327 mL 0.1634 mL 0.3269 mL 0.8172 mL
100 mM 0.0262 mL 0.1308 mL 0.2615 mL 0.6538 mL
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MX1013 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MX1013582316-00-5CV1013 Z-VD-FMKMX 1013MX-1013CV 1013CV-1013CaspaseApoptosisbrainischemia/strokecaspasecytoprotectiveliverfailuremyocardialinfarctionInhibitorinhibitorinhibit

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