1. Apoptosis
  2. Bcl-2 Family

WEHI-539 hydrochloride 

Cat. No.: HY-15607A Purity: 97.85%
Data Sheet SDS Handling Instructions

WEHI-539 hydrochloride is a selective inhibitor of Bcl-XL with IC50 of 1.1 nM.

For research use only. We do not sell to patients.
WEHI-539 hydrochloride Chemical Structure

WEHI-539 hydrochloride Chemical Structure

CAS No. : 2070018-33-4

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $164 In-stock
5 mg $120 In-stock
10 mg $150 In-stock
50 mg $520 In-stock
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Other Forms of WEHI-539 hydrochloride:

    WEHI-539 hydrochloride purchased from MCE. Usage Cited in: Int J Cancer. 2017 Sep 26.

    Evaluation of PARP protein cleavage in Mneo and MXL90 cells after treatment for 24h with 20 μM Cisplatin, in presence of increasing doses of the specific Bcl-xL inhibitor WEHI-539.

    WEHI-539 hydrochloride purchased from MCE. Usage Cited in: Blood. 2014 Dec 4;124(24):3587-96.

    CD41+ or CD41 cells are treated with DMSO as a vehicle control or a BCL-xL inhibitor (WEHI-539; 0.2, 0.4, and 0.8 μM) on OP9 stromal cells in the presence of THPO for 7 days. The number of viable cells is counted.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References


    WEHI-539 hydrochloride is a selective inhibitor of Bcl-XL with IC50 of 1.1 nM.

    IC50 & Target

    IC50: 1.1 nM (Bcl-XL)[1]

    In Vitro

    WEHI-539 hydrochloride is a selective inhibitor of Bcl-XL. WEHI-539 augments Carboplatin induced caspase 3/7 activity, PARP cleavage and annexin V labelling. WEHI-539 as a single agent causes noticeable PARP cleavage in Ovcar-4 (5 μM in Ovcar-4.) and Ovsaho (1 μM in Ovsaho) cells[2].

    Preparing Stock Solutions
    Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
    1 mM 1.6124 mL 8.0622 mL 16.1244 mL
    5 mM 0.3225 mL 1.6124 mL 3.2249 mL
    10 mM 0.1612 mL 0.8062 mL 1.6124 mL
    Cell Assay

    WEHI-539 (Medchem Express, NJ, USA) is dissolved in DMSO (20 mM) and stored, and then diluted with appropriate media before use[2].

    Ovcar-8, Ovcar-3, Ovcar-4 and Ovsaho cells are grown in the RPMI, Igrov-1, Cov-362 and Cov-318 cells are grown in DMEM and Fuov-1 cells are grown in DMEM/F-12 nutrient mixture. ABT-737, ABT-199 and WEHI-539 (Medchem Express, NJ, USA), are prepared as a 20 mM solution in DMSO. For cell growth assays, cells are plated in 96 wells plate (5,000 cells/well for all cell lines except Ovcar-8 which is plated at a density of 2,500 cells/well). The next day, cells are treated with drugs. After 72 h the culture medium is removed and the cells are fixed with 100 μL of cold 10 % Trichloroacetic acid (TCA), incubated on ice for 30 min and stained with 0.4 % sulforhodamine B (SRB). The data are analysed by using Graphpad Prism 4 software. Non-linear regression is used to fit a four parameters Hill equation. For drug combinations studies the cells are exposed simultaneously to a range of concentrations of carboplatin combined with fixed concentration of BH3 mimetics that is expected from the single agent studies to cause 5 % growth inhibition: ABT-737, 1 μM in Ovcar-8, Ovcar-3 and Igrov-1, 2 μM in Ovcar-4 and Ovsaho and 6 μM in Cov-362; ABT-199, 1 μM in Ovcar-4, 2 μM in Ovcar-3, Igrov-1, Cov-362 and Ovsaho and 3 μM in Ovcar-8; WEHI-539, 0.2 μM in Igrov-1, 0.3 μM in Ovcar-8, 1 μM in Ovcar-3 and Ovsaho, 3.1 μM in Cov-362 and 5 μM in Ovcar-4. Surviving cell number is assessed by SRB staining. A combination index (CI) is calculated[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.



    Please store the product under the recommended conditions in the Certificate of Analysis.


    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    10 mM in DMSO

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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