2. Metabolic Enzyme/Protease PI3K/Akt/mTOR NF-κB Apoptosis Autophagy
  3. MMP Akt NF-κB Proteasome Apoptosis Autophagy
  4. Isoginkgetin

Isoginkgetin is a pre-mRNA splicing inhibitor inhibitor. Isoginkgetin also inhibits activities of both Akt, NF-κB and MMP-9. Isoginkgetin inhibits the activity of the 20S proteasome, induces apoptosis and activates autophagy.

For research use only. We do not sell to patients.

Isoginkgetin Chemical Structure

Isoginkgetin Chemical Structure

CAS No. : 548-19-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 75 In-stock
Solution
10 mM * 1 mL in DMSO USD 75 In-stock
Solid
5 mg USD 60 In-stock
10 mg USD 100 In-stock
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Customer Review

Based on 15 publication(s) in Google Scholar

Isoginkgetin Related Antibodies

Top Publications Citing Use of Products

    Isoginkgetin purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Nov 29;27(23):8335.  [Abstract]

    Isoginkgetin (Iso; 10, 15, 25 µM; 24, 48, 72 h) reduces the viability of U87MG in a time and dose-dependent manner.

    Isoginkgetin purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Nov 29;27(23):8335.  [Abstract]

    Isoginkgetin (Iso; 15, 25 µM; 48 h) determines a redistribution of vimentin, which no longer appears distributed in the extensions but are thickened in a roundish shape in U87MG cells.

    Isoginkgetin purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Nov 29;27(23):8335.  [Abstract]

    The number of colonies decreased by 38% and 57%, respectively, in U87MG treated with Isoginkgetin (Iso; 15, 25 µM; 12 days) compared to the control cells.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Isoginkgetin is a pre-mRNA splicing inhibitor inhibitor. Isoginkgetin also inhibits activities of both Akt, NF-κB and MMP-9. Isoginkgetin inhibits the activity of the 20S proteasome, induces apoptosis and activates autophagy[1][2].

    In Vitro

    Isoginkgetin (0-20 μM, 24 h) inhibits cell invasion in a dose-dependent manner[1].
    Isoginkgetin (10 μM, 0-24 h) induces the accumulation of ubiquitinated protein cargo into perinuclear aggregates, activates autophagy and induces lysosomal swelling, repositioning, and acidification[2].
    Isoginkgetin (10 μM, 0-6 h) induces ER stress, impairs the unfolded protein response, and results in accumulation of ERAD substrates[2].
    Isoginkgetin directly inhibits the chymotrypsin-, trypsin-, and caspase-like activities of the 20S proteasome and impairs NF-κB signaling[2].
    Isoginkgetin (30 μM, 0-24 h) induces apoptosis in MM cell lines[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Isoginkgetin Related Antibodies

    Cell Invasion Assay[1]

    Cell Line: MDA-MB-231 and B16F10
    Concentration: 0, 5, 10 and 20 μM
    Incubation Time: 24 h
    Result: Inhibited cell invasion in a dose-dependent manner.

    Western Blot Analysis[2]

    Cell Line: HeLa cells and MM cell lines (MM1S, 8826, OPM2, H929, JJN3 and U266)
    Concentration: 10 μM in HeLa cells, 30 μM in MM cell lines
    Incubation Time: 0-24 h
    Result: Induced the expression of cleaved PARP-1 and Casp-3. Increased the protein levels of both ATF4 and ATF3. Decreased the level of LC3 I. Increased TFEB activation. Prevented the degradation of IκBα in response to stimulation with TNF-α.

    Cell Viability Assay[2]

    Cell Line: MM1S, 8826, OPM2, H929, JJN3 and U266 cells
    Concentration:
    Incubation Time: 72 h
    Result: Inhibited cell viability with IC50 values of 3.992, 3.716, 4.749, 2.564, 3.806 and 4.147 μM against MM1S, 8826, OPM2, H929, JJN3 and U266 cells, respectively.
    In Vivo

    Isoginkgetin (4 mg/kg; i.p.; daily for 14 days) shows anti-inflammatory effects[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Kunming mice (age 8–10weeks, 30–50 g)[3]
    Dosage: 4 mg/kg
    Administration: Daily for 14 days by intraperitoneally (i.p.) prior to LPS (0.83mg/kg) administration.
    Result: Markedly suppressed the production of IL-1β, IL-6, tumor necrosis factor-alpha, cyclooxygenase-2, inducible nitric oxide, and reactive oxygen species.
    Molecular Weight

    566.51

    Formula

    C32H22O10
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C=C(C2=CC=C(OC)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4OC)C(O)=CC(O)=C13
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (176.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7652 mL 8.8260 mL 17.6519 mL
    5 mM 0.3530 mL 1.7652 mL 3.5304 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.67 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.08 mg/mL (3.67 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 1.67 mg/mL (2.95 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.95%

    COA (260 KB) HNMR (281 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7652 mL 8.8260 mL 17.6519 mL 44.1298 mL
    5 mM 0.3530 mL 1.7652 mL 3.5304 mL 8.8260 mL
    10 mM 0.1765 mL 0.8826 mL 1.7652 mL 4.4130 mL
    15 mM 0.1177 mL 0.5884 mL 1.1768 mL 2.9420 mL
    20 mM 0.0883 mL 0.4413 mL 0.8826 mL 2.2065 mL
    25 mM 0.0706 mL 0.3530 mL 0.7061 mL 1.7652 mL
    30 mM 0.0588 mL 0.2942 mL 0.5884 mL 1.4710 mL
    40 mM 0.0441 mL 0.2206 mL 0.4413 mL 1.1032 mL
    50 mM 0.0353 mL 0.1765 mL 0.3530 mL 0.8826 mL
    60 mM 0.0294 mL 0.1471 mL 0.2942 mL 0.7355 mL
    80 mM 0.0221 mL 0.1103 mL 0.2206 mL 0.5516 mL
    100 mM 0.0177 mL 0.0883 mL 0.1765 mL 0.4413 mL
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    Isoginkgetin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Isoginkgetin548-19-6MMPAktNF-κBProteasomeApoptosisAutophagyMatrix metalloproteinasesPKBProtein kinase BNuclear factor-κBNuclear factor-kappaBHT1080MDA-MB-231HeLaanticancerInhibitorinhibitorinhibit

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