2. Academic Validation
  3. Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells

Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells

  • Mediators Inflamm. 2019 Jun 17;2019:7162976. doi: 10.1155/2019/7162976.
Zhimei Wang 1,  Feng Wang 1,  Xiangquan Kong 1,  Xiaofei Gao 1,  Yue Gu 1,  Junjie Zhang 1
Affiliations

Affiliation

  • 1 Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
PMID: 31316302 DOI: 10.1155/2019/7162976
Abstract

Background: Oscillatory shear stress (OSS) disrupts endothelial homeostasis and promotes oxidative stress, which can lead to atherosclerosis. In atherosclerotic lesions, Toll-like Receptor 4 (TLR4) is highly expressed. However, the molecular mechanism by which TLR4 modulates oxidative changes and the cell signaling transudation upon OSS is yet to be determined.

Methods and results: Carotid artery constriction (CAC) surgery and a parallel-plate flow chamber were used to modulate shear stress. The results showed that OSS significantly increased the oxidative burden, and this was partly due to TLR4 activation. OSS activated NOX2 and had no significant influence to NOX1 or NOX4 in endothelial cells (ECs). OSS phosphorylated caveolin-1, promoted its binding with endothelial nitric oxide synthase (eNOS), and resulted in deactivation of eNOS. TLR4 inhibition restored levels of nitric oxide (NO) and superoxide dismutase (SOD) in OSS-exposed cells.

Conclusion: TLR4 modulates OSS-induced oxidative stress by activating NOX2 and suppressing eNOS.

Figures
Products