2. Immunology/Inflammation
  3. Aryl Hydrocarbon Receptor
  4. ITE

ITE is a potent endogenous agonist of aryl hydrocarbon receptor (AhR), binding directly to AHR, with a Ki of 3 nM. ITE also has immunosuppressive activity.

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CAS 番号 : 448906-42-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 在庫あり
Solution
10 mM * 1 mL in DMSO USD 77 在庫あり
Solid
1 mg $33 在庫あり
5 mg $70 在庫あり
10 mg $120 在庫あり
25 mg $253 在庫あり
50 mg $380 在庫あり
100 mg $600 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 8 publication(s) in Google Scholar

Other Forms of ITE:

ITE 関連抗体

Top Publications Citing Use of Products

    ITE purchased from MedChemExpress. Usage Cited in: Microbiome. 2023 Nov 7;11(1):245.  [Abstract]

    Images under fluorescence microscopy showing different regions in hippocampus. Iba1 staining (green) and nuclear staining (DIPA, blue). ITE treatment reversed the hippocampal pathology in IUGR rats.

    ITE purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2022 Dec 7;14(12):e15677.  [Abstract]

    Protein levels of AhR, p-SRC (Y416), and SRC, in the SK28R cell line after treatment with different AhR ligands for 24 h (5 μM BaP, 5 μM indirubin, 5 μM ITE, 10 nM TCDD and 5 μM FICZ).

    ITE purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2022 Dec 7;14(12):e15677.  [Abstract]

    Protein levels of AhR, p-SRC (Y416), and SRC, in the SK28R cell line after 24 h of treatment with increasing doses of ITE (0-5 μM).

    ITE purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2022 Dec 7;14(12):e15677.  [Abstract]

    Protein levels of the AhR, p-SRC (Y416), and SRC were analyzed by western blotting in SK28 after various times of treatment with ITE (5 μM) and quantified using ImageJ (Fiji).

    ITE purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2022 Dec 7;14(12):e15677.  [Abstract]

    Histogram showing the expression (RT-qPCR) of THBS1 and MMP1 in SK28 after treatment by ITE (5 μM) at different times.

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    製品説明

    ITE is a potent endogenous agonist of aryl hydrocarbon receptor (AhR), binding directly to AHR, with a Ki of 3 nM. ITE also has immunosuppressive activity.

    IC50 & Target

    Ki: 3 nM (AhR)[1]
    体外実験

    ITE is an endogenous agonist of AhR, binding directly to AHR, with a Ki of 3 nM[1]. ITE (0.03-30 mg/mL) decreases the antigen-specific T-cell proliferative responses[2]. ITE potently inhibits human pulmonary artery endothelial (HPAECs) growth at 10 and 20 μM, but shows no effect at 0.01-5 μM. ITE does not affect cell cycle progress of HPAECs at 10 and 20 μM, or induce expression of cleaved caspase-3 protein in HPAECs at 20 μM. In addition, ITE (20 μM) elevates CYP1A1 and CYP1B1 mRNA levels and decreases the levels of AhR protein in HPAECs[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ITE 関連抗体
    体内実験

    ITE (200 μg, i.p.) significantly suppresses the development of experimental autoimmune uveitis (EAU) in mice. ITE reduces the proportions of cells expressing IFN-γ, IL-17, or IL-10 in mice. ITE also suppresses the secretion of inflammatory cytokines by LN cells in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    286.31

    分子式

    C14H10N2O3S
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=CSC(C(C2=CNC3=C2C=CC=C3)=O)=N1)OC
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 62.5 mg/mL (218.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4927 mL 17.4636 mL 34.9272 mL
    5 mM 0.6985 mL 3.4927 mL 6.9854 mL
    10 mM 0.3493 mL 1.7464 mL 3.4927 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    一般には略語で表示されます：C1V1 = C2V2

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    純度とドキュメンテーション

    純度: 99.27%

    COA (252 KB) HNMR (185 KB) LCMS (140 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [3]

    Subconfluent cells (25, 000 cells/well) are seeded in 96-well plates. Cells are treated with ITE at 5, 10 and 20 µM or DMSO (0.1% v/v) in ECM for 2, 4 or 6 days with a change of ECM containing DMSO or ITE every other day (5 wells/treatment). At the end of treatment, cells are incubated with MTT reagent for 4 hr, and solubilized in crystal dissolving solution (100 µL/well) for 20 min. The absorbance is determined at 570 nm using the microplate reader[3].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [2]

    Mice[2]
    Eight- to 12-week-old female B10.A mice is used in the assay. Daily treatment starts on day 0 and consists of 200 μg of ITE suspended in 0.2 mL PBS, given intraperitoneally. Control mice are similarly treated with 0.2 mL of the vehicle, PBS containing 3.6% DMSO[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.4927 mL 17.4636 mL 34.9272 mL 87.3179 mL
    5 mM 0.6985 mL 3.4927 mL 6.9854 mL 17.4636 mL
    10 mM 0.3493 mL 1.7464 mL 3.4927 mL 8.7318 mL
    15 mM 0.2328 mL 1.1642 mL 2.3285 mL 5.8212 mL
    20 mM 0.1746 mL 0.8732 mL 1.7464 mL 4.3659 mL
    25 mM 0.1397 mL 0.6985 mL 1.3971 mL 3.4927 mL
    30 mM 0.1164 mL 0.5821 mL 1.1642 mL 2.9106 mL
    40 mM 0.0873 mL 0.4366 mL 0.8732 mL 2.1829 mL
    50 mM 0.0699 mL 0.3493 mL 0.6985 mL 1.7464 mL
    60 mM 0.0582 mL 0.2911 mL 0.5821 mL 1.4553 mL
    80 mM 0.0437 mL 0.2183 mL 0.4366 mL 1.0915 mL
    100 mM 0.0349 mL 0.1746 mL 0.3493 mL 0.8732 mL
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    ITE Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ITE448906-42-1Aryl Hydrocarbon ReceptorAhRInhibitorinhibitorinhibit

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    製品名:
    ITE
    製品番号:
    HY-19317
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