MQAE

Name: MQAE
Brand: MedChemExpress
SKU: HY-D0090
Rating: 5.0 (8 reviews)

Cat. No.: HY-D0090 Purity: 99.84%: FAQs
Data Sheet SDS COA Handling Instructions

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MQAE is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. MQAE can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.

For research use only. We do not sell to patients.

MQAE Chemical Structure

CAS No. : 162558-52-3

Size	Price	Stock	Quantity
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50 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 156	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 336	In-stock	Estimated Time of Arrival: December 31
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Based on 8 publication(s) in Google Scholar

7 Publications Citing Use of MCE MQAE

•CCS Chemistry. 20 Jul 2021.

: MQAE purchased from MedChemExpress. Usage Cited in: Front Nutr. 2021 Mar 29;8:638390. [Abstract]; Nile Red, MQAE, and CoroNa™ Green are used to stain the triglycerides (TG), chloride ions (Cl-), and sodium ions (Na+), respectively.

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Description

In Vitro

General Protocol

1 Preparation of MQAE working solution
1.1 Preparation of the stock solution
Dissolve 1 mg MQAE in 0.3066 mL DMSO to obtain 10 mM of MQAE.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of MQAE working solution
Dilute the stock solution in Krebs-hepes buffer (20 mM HEPES, 128 mM NaCl, 2.5 mM KCl, 2.7 mM CaCl₂, 1 mM MgCl₂, 16 mM glucose, pH 7.4) to obtain 5-10 mM of MQAE working solution.
Note: Please adjust the concentration of MQAE working solution according to the actual situation.

2 Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of MQAE working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS.

3 Storage
-20℃, 1 year. Protect from light.
4 Precautions
4.1 It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
4.2 Please adjust the concentration of MQAE working solution according to the actual situation.
4.3 This product is for R&D use only, not for drug, household, or other uses.
4.4 For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

326.19

Formula

C₁₄H₁₆BrNO₃

CAS No.

162558-52-3

Appearance

Solid

Color

Light yellow to khaki

Emission (Em)

460

Excitation (Ex)

355

SMILES

COC1=CC2=CC=C[N+](CC(OCC)=O)=C2C=C1.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (306.57 mM; Need ultrasonic)

DMSO : ≥ 35 mg/mL (107.30 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0657 mL	15.3285 mL	30.6570 mL
5 mM	0.6131 mL	3.0657 mL	6.1314 mL
10 mM	0.3066 mL	1.5328 mL	3.0657 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (6.38 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (306.57 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.84%

Select Batch:

Data Sheet (278 KB) SDS (0 KB)

COA (248 KB) HNMR (302 KB) LCMS (119 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: MQAE is a fluorescent indicator for specifically stain intracellular chloride ions (Cl⁻) with blue fluorescence.
Method: For cell staining.
1. Freshly prepared tuna colloidal particle solutions (1 mL) are mixed with MQAE (40 µL; 30 min; dark).
2. Stained samples (10 µL) are added onto glass microslides and square cover glasses are placed onto the samples.
3. Finally, these samples are observed using a laser confocal microscopy microscope (Zeiss LSM 710, Carl Zeiss, Jena, Germany).

References

[1]. Kovalchuk Y, et al. Two-photon chloride imaging using MQAE in vitro and in vivo. Cold Spring Harb Protoc. 2012 Jul 1;2012(7):778-85. [Content Brief]

[2]. Andersson C, et al. Determination of chloride efflux by X-ray microanalysis versus MQAE-fluorescence. Microsc Res Tech. 2 [Content Brief]

[3]. Koncz C, et al. Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am J Physiol. 1994 Dec;267(6 Pt 2):H2114-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.0657 mL	15.3285 mL	30.6570 mL	76.6424 mL
	5 mM	0.6131 mL	3.0657 mL	6.1314 mL	15.3285 mL
	10 mM	0.3066 mL	1.5328 mL	3.0657 mL	7.6642 mL
	15 mM	0.2044 mL	1.0219 mL	2.0438 mL	5.1095 mL
	20 mM	0.1533 mL	0.7664 mL	1.5328 mL	3.8321 mL
	25 mM	0.1226 mL	0.6131 mL	1.2263 mL	3.0657 mL
	30 mM	0.1022 mL	0.5109 mL	1.0219 mL	2.5547 mL
	40 mM	0.0766 mL	0.3832 mL	0.7664 mL	1.9161 mL
	50 mM	0.0613 mL	0.3066 mL	0.6131 mL	1.5328 mL
	60 mM	0.0511 mL	0.2555 mL	0.5109 mL	1.2774 mL
	80 mM	0.0383 mL	0.1916 mL	0.3832 mL	0.9580 mL
	100 mM	0.0307 mL	0.1533 mL	0.3066 mL	0.7664 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

MQAE Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MQAE162558-52-3Fluorescent DyeInhibitorinhibitorinhibit

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MQAE

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MQAE Related Antibodies

7 Publications Citing Use of MCE MQAE

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MQAE Related Antibodies

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Complete Stock Solution Preparation Table

MQAE Related Classifications

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