1. MAPK/ERK Pathway
  2. MAP3K

NQDI-1 

Cat. No.: HY-19566 Purity: 98.04%
Handling Instructions

NQDI-1 inhibits apoptosis signal-regulating kinase 1 (ASK1) with a Ki of 500 nM.

For research use only. We do not sell to patients.
NQDI-1 Chemical Structure

NQDI-1 Chemical Structure

CAS No. : 175026-96-7

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10 mM * 1 mL in DMSO USD 79 In-stock
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10 mg USD 120 In-stock
50 mg USD 480 In-stock
100 mg USD 900 In-stock
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Description

NQDI-1 inhibits apoptosis signal-regulating kinase 1 (ASK1) with a Ki of 500 nM. IC50 value: 500 nM (Ki) [1] Target: ASK1 in vitro: NQDI-1 inhibits the activation of caspase-3 and prevents cell death by apoptosis. It is neuroprotective and prevents the ROS-induced apoptosis of the neurons. [2] ASK1 inhibitor NQDI-1 completely prevented caspaseactivation and cell death. 10 μM NQDI-1 reduced ASK1 phosphorylation to 40% of control and reduced the phosphorylation of JNK and p38. NQDI-1 at 30 μM almost completely and 50 μM completely prevented ASK1, JNK and p38 phosphorylation. We then tested the efficacy of both 25 μM and 50 μM on bone formation using the Matrigel/rhBMP ectopic ossification CD-1 mouse strain model. In this model, 25 μM increased bone volume, whereas 50 μM NQDI-1 decreased ossification, as compared with WT Alcian blue and alizarin red staining confirmed that both chondrogenesis and osteogenesis were enhanced.[3] in vivo: To confirm this result in C57BL/6 mice, we added 30 μM NQDI-1 to the BMP/Matrigel mixture before injection into WT mice and repeated the ectopic ossification experiments. After 2 weeks, this concentration of NQDI-1 was sufficient to increase bone volume 64% above control (P=0.04), which was comparable to the 82% increase observed in the KO. No differences were observed in the BMD of the treated and control ectopic masses. We also confirmed that both chondrogenesis and osteogenesis were enhanced, based on the increased alcian blue and alizarin red staining with respect to control. Finally, we determined the number of cells in each ectopic bone sample using DAPI staining. Both the KO mouse, and the WT with NQDI-1 showed increased cell counts in the bone forming tissue.[3]

References
Molecular Weight

319.31

Formula

C₁₉H₁₃NO₄

CAS No.

175026-96-7

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 10 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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NQDI-1
Cat. No.:
HY-19566
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