1. GPCR/G Protein
  2. Prostaglandin Receptor
  3. PF-04418948

PF-04418948 is an orally active, potent and selective prostaglandin EP2 receptor antagonist with an IC50 of 16 nM.

For research use only. We do not sell to patients.

PF-04418948 Chemical Structure

PF-04418948 Chemical Structure

CAS No. : 1078166-57-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 84 In-stock
Solution
10 mM * 1 mL in DMSO USD 84 In-stock
Solid
1 mg USD 36 In-stock
5 mg USD 77 In-stock
10 mg USD 115 In-stock
50 mg USD 484 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

    PF-04418948 purchased from MedChemExpress. Usage Cited in: J Virol. 2018 Sep 26;92(20):e01018-18.  [Abstract]

    A549 cells are added with an EP2 or an EP4 inhibitor. After 48 h, cells are subjected to WB with an anti-Axl antibody.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    PF-04418948 is an orally active, potent and selective prostaglandin EP2 receptor antagonist with an IC50 of 16 nM[1].

    IC50 & Target

    EP2

    16 nM (IC50)

    In Vitro

    PF-04418948 (2 μM; 90 min) inhibits prostaglandin E2 (PGE2)-induced increase in cAMP[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: CHO cells
    Concentration: 2 μM
    Incubation Time: 90 min
    Result: Inhibited prostaglandin E2 (PGE2)-induced increase in cAMP in cells expressing EP2 receptors with a functional KB value of 1.8 nM.
    In Vivo

    PF-04418948 (oral gavage; 1, 3, and 10 mg/kg; once) attenuats the butaprost-induced cutaneous blood flow response[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Sprague Dawley rats[1]
    Dosage: 1, 3, and 10 mg/kg
    Administration: Oral gavage; 1, 3, and 10 mg/kg; once
    Result: Reduced the peak and AUC butaprost-induced cutaneous blood flow response in a dose-dependent fashion.
    Clinical Trial
    Molecular Weight

    409.41

    Formula

    C23H20FNO5

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C1(COC2=CC=C3C=C(OC)C=CC3=C2)CN(C(C4=CC=C(F)C=C4)=O)C1)O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (122.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4425 mL 12.2127 mL 24.4254 mL
    5 mM 0.4885 mL 2.4425 mL 4.8851 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 6.5 mg/mL (15.88 mM); Clear solution

    • Protocol 2

      Add each solvent one by one:  2% DMSO    40% PEG300    5% Tween-80    53% Saline

      Solubility: ≥ 2.6 mg/mL (6.35 mM); Clear solution

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.56%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4425 mL 12.2127 mL 24.4254 mL 61.0635 mL
    5 mM 0.4885 mL 2.4425 mL 4.8851 mL 12.2127 mL
    10 mM 0.2443 mL 1.2213 mL 2.4425 mL 6.1063 mL
    15 mM 0.1628 mL 0.8142 mL 1.6284 mL 4.0709 mL
    20 mM 0.1221 mL 0.6106 mL 1.2213 mL 3.0532 mL
    25 mM 0.0977 mL 0.4885 mL 0.9770 mL 2.4425 mL
    30 mM 0.0814 mL 0.4071 mL 0.8142 mL 2.0354 mL
    40 mM 0.0611 mL 0.3053 mL 0.6106 mL 1.5266 mL
    50 mM 0.0489 mL 0.2443 mL 0.4885 mL 1.2213 mL
    60 mM 0.0407 mL 0.2035 mL 0.4071 mL 1.0177 mL
    80 mM 0.0305 mL 0.1527 mL 0.3053 mL 0.7633 mL
    100 mM 0.0244 mL 0.1221 mL 0.2443 mL 0.6106 mL
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    PF-04418948 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    PF-04418948
    Cat. No.:
    HY-18966
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