1. GPCR/G Protein
  2. Adiponectin Receptor

AdipoRon 

Cat. No.: HY-15848 Purity: 99.49%
Data Sheet SDS Handling Instructions

AdipoRon is a novel AdipoR agonist, binding to both AdipoR1 (Kd=1.8 μM) and AdipoR2 (Kd=3.1 μM).

For research use only. We do not sell to patients.
AdipoRon Chemical Structure

AdipoRon Chemical Structure

CAS No. : 924416-43-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $66 In-stock
10 mg $60 In-stock
50 mg $230 In-stock
100 mg $410 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Featured Recommendations

Related Screening Libraries:

View More Collapse
  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

AdipoRon is a novel AdipoR agonist, binding to both AdipoR1 (Kd=1.8 μM) and AdipoR2 (Kd=3.1 μM).

IC50 & Target

Kd: 1.8 μM (AdipoR1), 3.1 μM (AdipoR2)

In Vitro

AdipoRon (50 μM) attenuates the expression of TNF-α and TGF-β1, apparently in a dose-dependent manner in the L02 cells. AdipoRon exhibits significant and dosage-dependent growth suppression on macrophages[1]. AdipoRon treatment significantly improves cardiac functional recovery after reperfusion, and inhibits post-MI apoptosis[2]. AdipoRon exerts vasodilation by mechanisms distinct to adiponectin. AdipoRon induces vasorelaxation without a marked decrease in VSMC [Ca2+]i[3].

In Vivo

AdipoRon (0.02, 0.1, and 0.5 mg/kg, i.g.) alleviates D-GalN induced hepatotoxicity in mice. AdipoRon dose-dependently prevents hepatic architecture distortion against D-GalN challenge. The hepatoprotective potential of AdipoRon is particularly evident in higher dosages (0.1 and 0.5 mg/kg)[1]. Enhanced cardiomyocyte apoptosis in APN-deficient mice is rescued by AdipoRon (50 mg/kg, p.o.) administration. Antiapoptotic effect of AdipoRon is attenuated but not lost in AMPK-DN mice[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.3336 mL 11.6681 mL 23.3361 mL
5 mM 0.4667 mL 2.3336 mL 4.6672 mL
10 mM 0.2334 mL 1.1668 mL 2.3336 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

The effects of AdipoRon on the proliferation of parenchymal and non-parenchymal hepatocytes are evaluated in vitro via L02 and RAW264.7, by MTT assay as described with slight modification: 100 μL cells suspension (6×104/mL) are seeded in a 96-well plate and incubated for 18 h. Fresh media with AdipoRon are added at specified concentrations, and the incubations continue for a further 24 h. Then cells are incubated for 4 h with 0.5 mg/mL of MTT, and analyzed in a microplate reader at 490 nm. Each group is performed in six replications. The mean absorbance values corrected for a blank (medium only) are calculated as percentages of survival. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

AdipoRon is dissolved in DMSO and diluted by saline containing 0.5% sodium carboxymethyl cellulose (CMC-Na) [final vehicle: 5% DMSO (v/v) saline solution].

After 3 days of acclimation, mice are randomLy divided into six groups (9 mice in each): control, model, bicyclol (20 mg/kg), AdipoRon (0.02 mg/kg, 0.1 mg/kg, 0.5 mg/kg). The synthetic AdipoRon and bicyclol are dissolved in dimethylsulfoxide (DMSO) and diluted by saline containing 0.5% sodium carboxymethyl cellulose (CMC-Na) [final vehicle: 5% DMSO (v/v) saline solution]. All test groups are administered with vehicle (control and model groups) or therapeutic agents (bicyclol or AdipoRon groups) at a dosing volume of 10 mL/kg, by intragastric (i.g.) gavage twice per day for three consecutive days prior to D-GalN administration. 2 h after last treatment, mice are challenged with a single intraperitoneal (i.p.) administration of D-GalN saline solution at a dose of 600 mg/kg to induce acute liver injury, while the control group mice receive saline instead. The dosages are based on previous reports and preliminary experiment. Then mice are fasted for 20 h before orbital blood collection. Finally, all animals are sacrificed by cervical dislocation, and livers are harvested for biochemical or histopathology analysis. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

428.52

Formula

C₂₇H₂₈N₂O₃

CAS No.

924416-43-3

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 44 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number

 

Organization name *

Country *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
AdipoRon
Cat. No.:
HY-15848
Quantity: