1. Epigenetics
    TGF-beta/Smad
  2. PKC

Go 6983 (Synonyms: Goe 6983)

Cat. No.: HY-13689 Purity: >98.0%
Data Sheet SDS Handling Instructions

Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively.

For research use only. We do not sell to patients.
Go 6983 Chemical Structure

Go 6983 Chemical Structure

CAS No. : 133053-19-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $88 In-stock
5 mg $80 In-stock
10 mg $130 In-stock
50 mg $410 In-stock
100 mg $690 In-stock
200 mg   Get quote  
500 mg   Get quote  

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    Go 6983 purchased from MCE. Usage Cited in: Vet Microbiol. 2016 Aug 30;192:26-33.

    Effect of PKC inhibitor on NF-kB DNA binding activity and NFAT inhibitor on NFATc1 DNA binding activity. Lane N is negative control; lane 1 is control group; lane 2 is S. uberis group; lane 3 is pre-treated with 2 μM Go 6983; lane 4 is pre-treated with 5 μM Go 6983; lane 5 is pre-treated with 10 μM Go 6983.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively.

    IC50 & Target

    IC50: 7 nM (PKCα), 7 nM (PKCβ), 6 nM (PKCγ), 10 nM (PKCδ), 60 nM (PKCζ)

    In Vitro

    Go 6983 inhibits PKCμ with IC50 of 20 μM, and the pther PKC isoenzymes can be suppressed by Go 6983 with IC50 values from 7 to 60 nM[1]. Go 6983 (100 nM) significantly reduces PMN adherence to the endothelium and infiltration into the myocardium compared with I/R + PMN hearts, and significantly inhibits superoxide release from PMNs by 90 +/- 2% in rat hearts[2]. Go 6983 (200 nM) has a reduced cardioprotective effect compared with the cardioprotective Go 6983 concentrations (50 and 100 nM) despite inhibiting PMN superoxide release by 99%[3].

    References
    Preparing Stock Solutions
    Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
    1 mM 2.2598 mL 11.2992 mL 22.5984 mL
    5 mM 0.4520 mL 2.2598 mL 4.5197 mL
    10 mM 0.2260 mL 1.1299 mL 2.2598 mL
    Kinase Assay
    [1]

    Phosphorylation reactions are carried out in a total volume of 100 μL, containing buffer C (50 mM Tris-HCl, pH 7.5, 10 mM β-mercaptoethanol), 4 mM MgCl2, 10 μg PS, 100 nM TPA, 5 μL of a Sf158 cell extract as a source of recombinant PKCμ or of Sf9 cell extracts as a source of other recombinant PKC isoenzymes, 10 μg of syntide 2 as substrate, and 35 μM ATP containing 1 μCi [γ-32P]ATP. In some experiments, PS and TPA are omitted or various inhibitors at concentrations indicated in the text are added. After incubation for 10 min at 30°C, the reaction is terminated by transferring 50 μL of the assay mixture onto a 20 mm square piece of phosphocellulose paper, which is washed 3 times in deionized water and twice in acetone. The radioactivity on each paper is determined by liquid scintillation counting. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    442.51

    Formula

    C₂₆H₂₆N₄O₃

    CAS No.

    133053-19-7

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 34 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: >98.0%

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    Inquiry Information

    Product Name:
    Go 6983
    Cat. No.:
    HY-13689
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