2. PI3K/Akt/mTOR Stem Cell/Wnt
  3. GSK-3
  4. Tideglusib

Tideglusib (NP031112) is an irreversible GSK-3 inhibitor with IC50s of 5 nM and 60 nM for GSK-3βWT (1 h preincubation) and GSK-3βC199A (1 h preincubation), respectively.

For research use only. We do not sell to patients.

CAS No. : 865854-05-3

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10 mM * 1 mL in DMSO
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Customer Review

Based on 7 publication(s) in Google Scholar

Tideglusib Related Antibodies

Top Publications Citing Use of Products

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Western blot analysis of p-GSK3β and GSK3β in BEAS-2B and MLE-12 cells treated under indicated conditions: No EF, EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h)+Tideglusib (TID, 20 µM, 24 h), with quantification of p-GSK3β/GSK3β ratio.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Western blot analysis of p-GSK3β and GSK3β in BEAS-2B and MLE-12 cells treated under indicated conditions: No EF, EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h)+Tideglusib (TID, 20 µM, 24 h), with quantification of p-GSK3β/GSK3β ratio.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Immunofluorescence analysis of Ki-67 in MLE-12 cells treated under indicated conditions: No EF, Tideglusib (TID; 20 µM, 24 h), EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h)+Tideglusib (TID; 20 µM, 24 h), with quantification of Ki-67 positive cells.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Immunofluorescence analysis of Ki-67 in BEAS2B cells treated under indicated conditions: No EF, Tideglusib (TID; 20 μM, 24 h), EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h) + TID(20 μM, 24 h), with quantification of Ki-67 positive cells.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 30:242:275-287.  [Abstract]

    HuH7 cells were treated with 7,8-DHF (5 μM), Tideglusib (10 μM) and concentration gradient RSL3 (0-10 μM) for 24 h. Cell viability was measured by CCK-8 assay.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Int J Clin Exp Pathol. 2017;10(3):3033-3042.

    Response of 6 HCC cell lines to 12 antitumor agents (Tideglusib; 0-10μM). The IC50 values were determined by MTS assay and calculated with the aid of GraphPad Prism 5.0 software by nonlinear regression.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Int J Clin Exp Pathol. 2017;10(3):3033-3042.

    Response of 6 HCC cell lines to 12 antitumor agents (Tideglusib; 0-10μM).

    View All GSK-3 Isoform Specific Products:

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    GSK-3 GSK-3α GSK-3β

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Tideglusib (NP031112) is an irreversible GSK-3 inhibitor with IC50s of 5 nM and 60 nM for GSK-3βWT (1 h preincubation) and GSK-3βC199A (1 h preincubation), respectively.

    IC50 & Target[1]

    GSK-3β(WT)

    5 nM (IC50)

    GSK-3β(C199A)

    60 nM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    Vero C1008 IC50
    0.67 μM
    Compound: 13
    Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as reduction in cell growth
    Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as reduction in cell growth
    		[PMID: 37244162]
    In Vitro

    Incubation of both astrocyte and microglial cultures with Tideglusib (NP031112) completely abrogates the induction of TNF-α and COX-2 expression after glutamate treatment. These effects of NP031112 are not caused by a loss of cell viability, because the 24 h exposure of astrocyte and microglial cells to this TDZD does not modify cell viability[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tideglusib Related Antibodies
    In Vivo

    Injection of Tideglusib (NP031112) (50 mg/kg) into the rat hippocampus dramatically reduces kainic acid-induced inflammation, as measured by edema formation using T2-weighted magnetic resonance imaging and glial activation and has a neuroprotective effect in the damaged areas of the hippocampus[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    334.39

    Formula

    C19H14N2O2S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(N(SC1=O)C2=C3C=CC=CC3=CC=C2)N1CC4=CC=CC=C4
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (99.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9905 mL 14.9526 mL 29.9052 mL
    5 mM 0.5981 mL 2.9905 mL 5.9810 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.70%

    SDS (396 KB)

    COA (262 KB) HNMR (266 KB) RP-HPLC (222 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [2]

    Rats[2]
    Adult male Wistar rats (8-12 weeks old) are used in this study. Rats (n≥5 per group) are placed into a stereotaxic apparatus. KA (1 μg in 2.5 μL PBS) alone or in combination with Tideglusib (2 ng in 2.5 μL PBS) is injected into the hippocampus. Control animals of the same age are injected with vehicle.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9905 mL 14.9526 mL 29.9052 mL 74.7630 mL
    5 mM 0.5981 mL 2.9905 mL 5.9810 mL 14.9526 mL
    10 mM 0.2991 mL 1.4953 mL 2.9905 mL 7.4763 mL
    15 mM 0.1994 mL 0.9968 mL 1.9937 mL 4.9842 mL
    20 mM 0.1495 mL 0.7476 mL 1.4953 mL 3.7381 mL
    25 mM 0.1196 mL 0.5981 mL 1.1962 mL 2.9905 mL
    30 mM 0.0997 mL 0.4984 mL 0.9968 mL 2.4921 mL
    40 mM 0.0748 mL 0.3738 mL 0.7476 mL 1.8691 mL
    50 mM 0.0598 mL 0.2991 mL 0.5981 mL 1.4953 mL
    60 mM 0.0498 mL 0.2492 mL 0.4984 mL 1.2460 mL
    80 mM 0.0374 mL 0.1869 mL 0.3738 mL 0.9345 mL
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    Tideglusib Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tideglusib865854-05-3NP031112NP 031112NP-031112GSK-3Glycogen synthase kinase-3Glycogen synthase kinase 3Inhibitorinhibitorinhibit

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