1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis

Actinomycin D (Synonyms: Dactinomycin; Actinomycin IV)

Cat. No.: HY-17559 Purity: 99.89%
Data Sheet SDS Handling Instructions

Actinomycin D inhibits DNA repair with IC50 of 0.42 μM.

For research use only. We do not sell to patients.
Actinomycin D Chemical Structure

Actinomycin D Chemical Structure

CAS No. : 50-76-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO $124 In-stock
5 mg $52 In-stock
10 mg $90 In-stock
50 mg $395 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Actinomycin D inhibits DNA repair with IC50 of 0.42 μM.

IC50 & Target

IC50: 0.42 μM (DNA repair)[1]

In Vitro

Actinomycin D is an inhibitor of DNA transcription and replication[1]. Actinomycin D markedly reduces the vascular smooth muscle cells (SMC) proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. Actinomycin D is extremely potent with an inhibitory concentration IC50 at 0.4 nM, whereas the lethal dose LD50 is at 260 microM. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by Actinomycin D. Extracellular signal-regulated kinases (Erk) involved in cell-cycle arrest are found to increase by Actinomycin D[2].

In Vivo

The pluronic gel containing 80 nM and 80μM Actinomycin D is applied topically to surround the rat carotid adventitia, the thickness of neointima is substantially reduced (45 and 55%, respectively)[2]. Mice in the Actinomycin D and fludarabine group lives significantly longer than the control group with P values of <0.001 and 0.007, respectively. Interestingly, single treatment with Actinomycin D is superior to fludarabine regarding overall survival (P=0.026)[3].

Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 0.7965 mL 3.9827 mL 7.9655 mL
5 mM 0.1593 mL 0.7965 mL 1.5931 mL
10 mM 0.0797 mL 0.3983 mL 0.7965 mL
Kinase Assay

Actinomycin D is co-incubated for 3 h at 30°C with a reaction mixture containing: 120 mg of a whole-cell extract of HeLa cells, 70 mM KCl, 0.4 mM each of dGTP, dCTP, dATP, and digoxygenylated-dUTP in reaction buffer containing 40mM Hepes-KOH (pH 7.6), 5 mM MgCl2, 0.5 mM Dithiotreitol, 2 mM EGTA, 10 mM phosphocreatine, 50 mg/mL creatine phosphate, and 360 mg/mL of bovine serum albumin. During this reaction, DNA damage is recognized and the excised patches are replaced by neosynthesized DNA fragments. Throughout this DNA synthesis, digoxygenylated-dUMPs are incorporated. The DNA repair reaction is stopped by three washes[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

Actinomycin D is prepared in phosphate-buffered saline (PBS) (Mice)[3].

The original Eμ-TCL1a transgenic mice have been backcrossed to C57BL/6 mice for >9 generations.The C57BL/6 wild-type mice are engrafted with tumor cells from Eμ-TCL-1 transgenic mice. The percentage of CD5+/CD19+ cells in the peripheral blood is routinely checked in mice by taking blood from the tail vein and analyzing it via flow cytometry. When the percentage of tumor cells in the peripheral blood reached 40-60%, treatment is started. Actinomycin D (0.06 mg/kg by 10 days) is applied daily via i.p. injections. MCE has not independently confirmed the accuracy of these methods. They are for reference only.







Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 27 mg/mL; DMSO: 250 mg/mL (Need ultrasonic); Methanol: 6.2 mg/mL; Methanol: 20 mg/mL (Need ultrasonic); H2O: < 12.5 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 99.89%

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