2. Neuronal Signaling
  3. Amyloid-β
  4. Thioflavin T

Thioflavin T  (Synonyms: Basic Yellow 1)

Cat. No.: HY-D0218 Purity: 99.40%
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Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. Excitation max.: ~385 nm (free); ~450 nm (bound); Emission max.: ~445 nm (free); ~485 nm (bound).

For research use only. We do not sell to patients.

CAS No. : 2390-54-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
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Customer Review

Based on 30 publication(s) in Google Scholar

Other Forms of Thioflavin T:

Thioflavin T Related Antibodies

Top Publications Citing Use of Products

30 Publications Citing Use of MCE Thioflavin T

Bio/Physico-chemical Assay
IF
Cell Imaging/Staining

    Thioflavin T purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 29:e01977.  [Abstract]

    Gastric cancer tissue samples (either EBER+ or EBER‐) were collected for immunofluorescence and thioflavin T (final concentration of 400 mM, incubated in the dark for 30 min) staining. Co‐localization of Cy3‐labeled EBNA1 with thioflavin T green fluorescence was observed by fluorescence microscopy. Cy3 labels EBNA1; Thioflavin T: green fluorescence, labels amyloid aggregates; DAPI: labels nuclei. Scale bar: 20 µm.

    Thioflavin T purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan 10:e2405054.  [Abstract]

    Evaluation of the time (0-30 h)‐dependent fluorescence evolution of ThT in the presence of CsgA or CsgA‐GHK, demonstrating the successful formation of fibrils. Insert indicates the fluorescence spectra. A plain buffer was used as a control. (ThT concentration: 1 × 10−2 mM).

    Thioflavin T purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Mar 17;13(1):1444.  [Abstract]

    ThT (right panel) fluorescence emission spectra of PQS-1017, PQS-1370, and PQS-1585 RNA under KCl or LiCl conditions. The excitation wavelength of ThT is 425 nm, and the emission spectrum of ThT is collected in the range of 450 nm to 700 nm.

    Thioflavin T purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Mar 17;13(1):1444.  [Abstract]

    ThT fluorescence emission spectra of PQS-675-WT, PQS-675-Mut, and PQS-1281-WT RNA under KCl or LiCl conditions. The excitation wavelength of ThT is 425 nm, and the emission spectrum of ThT is collected in the range of 450 nm to 700 nm.

    Thioflavin T purchased from MedChemExpress. Usage Cited in: Theranostics. 2022 Feb 28;12(6):2549-2559.  [Abstract]

    Fluorescence increment of probe AH-2 (at 587 nm) in comparison to ThT (at 480 nm) in response to Aβ fibrils. Each probe was kept at 2, 4, or 8 µM; Aβ fibrils were kept at 2 µM. F was the intensity of the probe treated with Aβ fibrils; while F0 was the probe intensity at the indicated concentration without Aβ fibrils treatment.

    Thioflavin T purchased from MedChemExpress. Usage Cited in: FEBS Open Bio. 2020 Oct;10(10):2122-2136.  [Abstract]

    The intracellular ERS analysis by ThT (0-10 μM, 20 min) assay under a fluorescence microscope and mean ThT fluorescence intensities were presented.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. Excitation max.: ~385 nm (free); ~450 nm (bound); Emission max.: ~445 nm (free); ~485 nm (bound)[1][2][3].

    In Vitro

    Thioflavin T is a fluorescent dye that can bind to β-fold-rich structures and is often used to measure protein fibrillation. Once bound, the emission spectrum of Thioflavin T will be red-shifted and the fluorescence intensity will increase, which can also be used to detect fibril formation. Here is a reference protocol for Aβ fibrils detection[2][3]:
    Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
    1. Prepare a 1 mM Thioflavin T stock solution in distilled water.
    2. Prepare a mixed sample containing LMW Aβ42 (5.5 μM), Thioflavin T solution (20 μM), and NaCl (100 mM).
    3. The mixtures were prepared in a 96-well half-area microplate. Seal the well plate and place it in a shaking incubator at 37°C for indicated time.
    4. Shake for 20 seconds before testing. Set the excitation light to 450 nm and the emission light to 485 nm. Read the fluorescence value using a microplate reader.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Thioflavin T Related Antibodies
    Molecular Weight

    318.86

    Formula

    C17H19ClN2S
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    CC1=CC=C2[N+](C)=C(C3=CC=C(N(C)C)C=C3)SC2=C1.[Cl-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 16.67 mg/mL (52.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (15.68 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1362 mL 15.6809 mL 31.3617 mL
    5 mM 0.6272 mL 3.1362 mL 6.2723 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.40%

    SDS (726 KB)

    COA (247 KB) HNMR (242 KB) RP-HPLC (227 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Kinase Assay
    [1]

    Thioflavin T (ThT) is prepared by dissolving ~3 mg dry powder in 1 mL water. The solution is filtered through 0.22 μm syringe filters followed by measurement of the concentration by diluting the stock solution in ethanol and using an extinction coefficient of 26,620 M-1 cm-1 at 416 nm. The stock solution is stored at 4 °C covered with foil and used for up to a month to make assay solutions by diluting either in water or desired buffer[1].
    Thioflavin T (ThT) fluorescence emission is measured with excitation at 450 nm and recording the spectrum between 465 and 565 nm with 5 nm slits using a FluoroMax 2 spectrofluorometer. The excitation spectra are collected by setting the emission wavelength to 482 nm and collecting the spectrum between 300 and 470 nm with 5 nm slit widths, and 1 s integration time and 1 nm interval. Emission spectra between 465 and 565 nm are collected upon excitation at 450 nm. Excitation and emission spectra in the presence of amyloid fibrils are measured with varying concentrations of Thioflavin T and 5 ng/mL amyloid (calculated based on starting protein concentration) before collecting the spectra[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.1362 mL 15.6809 mL 31.3617 mL 78.4043 mL
    5 mM 0.6272 mL 3.1362 mL 6.2723 mL 15.6809 mL
    10 mM 0.3136 mL 1.5681 mL 3.1362 mL 7.8404 mL
    15 mM 0.2091 mL 1.0454 mL 2.0908 mL 5.2270 mL
    DMSO 20 mM 0.1568 mL 0.7840 mL 1.5681 mL 3.9202 mL
    25 mM 0.1254 mL 0.6272 mL 1.2545 mL 3.1362 mL
    30 mM 0.1045 mL 0.5227 mL 1.0454 mL 2.6135 mL
    40 mM 0.0784 mL 0.3920 mL 0.7840 mL 1.9601 mL
    50 mM 0.0627 mL 0.3136 mL 0.6272 mL 1.5681 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Thioflavin T Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Thioflavin T2390-54-7Basic Yellow 1Basic Yellow1Basic Yellow-1Amyloid-ββ-amyloid peptideAβAbetaInhibitorinhibitorinhibit

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