2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related
  3. DNA/RNA Synthesis ADC Cytotoxin
  4. α-Amanitin

α-Amanitin  (Synonyms: α-Amatoxin)

Cat. No.: HY-19610 Purity: 96.33%
COA Handling Instructions

α-Amanitin is the principal toxin of several deadly poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.

For research use only. We do not sell to patients.

α-Amanitin Chemical Structure

α-Amanitin Chemical Structure

CAS No. : 23109-05-9

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Customer Review

Based on 27 publication(s) in Google Scholar

Other Forms of α-Amanitin:

α-Amanitin Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

α-Amanitin is the principal toxin of several deadly poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.

IC50 & Target

Traditional Cytotoxic Agents

 

In Vitro

α-Amanitin decreases TAF15 mRNA and TAF15 protein levels in MKN45 cells, and inhibits the RNAPII activity towards TAF15 mRNA[2]. alpha-Amanitin decreases cell viability by 14%, 21%, 41%, 44%, and 50% at concentrations of 100, 10, 1, 0.1, and 0.01 μg/mL, respectively. The LD50 of the alpha-Amanitin at 36 h is measured as 1 μg/mL. The total amount of protein within the cell at 24 h is significantly increased for the 1 μg/mL dose of alpha-Amanitin compared to the control[3]. α-Amanitin dramatically decreases the expression of gap junctional genes (Gja1, Gja4 and Gjc1) and FSHr and LHr in cumulus cells[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

α-Amanitin Related Antibodies
In Vivo

The intravenous LD50 dose of alpha-Amanitin is 0.327 mg/kg body weight after intravenous injection into BALB/c mice. After 12 h of alpha-Amanitin injection in caudal vein, the levels of WBC, RBC and HGB decrease significantly, while those of BUN and Crea increase significantly in serum. alpha-Amanitin inhibits some genes (Hsp90b1, Irx4, etc.), whose encoded proteins regulate the RNA polymerase II activity. alpha-Amanitin down-regulates some proteins (Nmi, Trpc5, etc.) taking part in the transcription progress[1]. alpha-Amanitin has potent activity in DTC suppression. Mice injected with alpha-Amanitin (0.4 mg/kg, i.p.)-treated cells maintain their body weight, while those receiving a peritoneal injection of MKN45 cells show a constant decrease in body weight[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

918.97

Appearance

Solid

Formula

C39H54N10O14S
CAS No.
SMILES

O=[S@](C[C@@](NC(CNC([C@@]([C@@H](C)CC)([H])N1)=O)=O)([H])C(N[C@@](C(N(C[C@H](O)C2)[C@]2([H])C3=O)=O)([H])CC(N)=O)=O)C4=C(C[C@](C(NCC1=O)=O)([H])NC([C@](N3)([H])[C@@H](C)[C@@H](O)CO)=O)C5=CC=C(O)C=C5N4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (108.82 mM; Need ultrasonic)

DMSO : 50 mg/mL (54.41 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0882 mL 5.4409 mL 10.8817 mL
5 mM 0.2176 mL 1.0882 mL 2.1763 mL
10 mM 0.1088 mL 0.5441 mL 1.0882 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 25 mg/mL (27.20 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.92%

COA (257 KB) LCMS (261 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[3]

The MTT assay is used to evaluate the overall functional integrity and viability of the cultured cells. The MCF-7 cells are put into 96-well plates (2×104 for each well), which are incubated for 24 h. The specific concentrations of alpha-Amanitin and β-Amanitin are added to the cell culture medium, and plates are incubated for an additional 36 h. MTT solution (1:10 ratio) and dimethyl sulfoxide (DMSO) (100 µL) are then added to the cell culture medium and plates are incubated overnight. The absorbance is measured at 570 nm on a plate reader. This experiment is repeated 3 times. The absorbance data are calculated as percentages according to the control group.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

For tumorigenicity tests, six colonies (untreated) and DTCs derived from MKN45 cells are individually injected subcutaneously into the left and right side of the backs of six 6-week-old female nude mice (BALB/cAjcl-nu/nu). These mice are monitored for 49 days after the inoculation or until tumors reach 10 mm in the largest diameter, and are then euthanized. For the PC model, 1.0×106 MKN45 cells are injected intraperitoneally into six 6-week-old female nude mice (BALB/cAjcl-nu/nu). Mice are then treated with CIS (4.0 mg/kg, intraperitoneal administration) or a combination of CIS and alpha-Amanitin (0.4 mg/kg, intraperitoneal administration). For the combination treatment, alpha-Amanitin is given 24 hours before CIS. Body weight is monitored for 28 days after the treatment.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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α-Amanitin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

α-Amanitin23109-05-9α-AmatoxinDNA/RNA SynthesisADC CytotoxinADC PayloadInhibitorinhibitorinhibit

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