Bicine
Based on 1 Customer Validation
Bicine is a buffer used in physiological research. Bicine is a derivative of the simple amino acid glycine that increases the sensitivity of guanosine enzyme histochemical detection.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 150-25-4
- Formula: C6H13NO4
- Molecular Weight:163.17
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Preparation of sections for histochemistry of guanine oxidase in human tissues[2]:
1. Tissue samples were snap frozen in liquid nitrogen and 10 µM thick cryostat sections were prepared.
2. After fixation with 2.5% glutaraldehyde in 0.05 M sodium cacodylate buffer (pH 7.4) at room temperature for 1 hour, sections were rinsed in Bicine buffer (0.1 M, pH 7.8) and incubated with xanthine oxidase at 37°C for 15 minutes, rinsed again in Bicine buffer and incubated at 37°C for 1-2 hours.
3. After rinsing in distilled water, sections were allowed to air dry, dehydrated with graded ethanol, transferred to xylene and mounted in synthetic resin.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 150-25-4
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Appearance Solid
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Molecular Weight 163.17
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Formula C6H13NO4
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Color White to off-white
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SMILES
O=C(O)CN(CCO)CCO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
H2O : 166.67 mg/mL (1021.45 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (306.43 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (270 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.1286 mL | 30.6429 mL | 61.2858 mL | 153.2144 mL |
| 5 mM | 1.2257 mL | 6.1286 mL | 12.2572 mL | 30.6429 mL | |
| 10 mM | 0.6129 mL | 3.0643 mL | 6.1286 mL | 15.3214 mL | |
| 15 mM | 0.4086 mL | 2.0429 mL | 4.0857 mL | 10.2143 mL | |
| 20 mM | 0.3064 mL | 1.5321 mL | 3.0643 mL | 7.6607 mL | |
| 25 mM | 0.2451 mL | 1.2257 mL | 2.4514 mL | 6.1286 mL | |
| 30 mM | 0.2043 mL | 1.0214 mL | 2.0429 mL | 5.1071 mL | |
| 40 mM | 0.1532 mL | 0.7661 mL | 1.5321 mL | 3.8304 mL | |
| 50 mM | 0.1226 mL | 0.6129 mL | 1.2257 mL | 3.0643 mL | |
| 60 mM | 0.1021 mL | 0.5107 mL | 1.0214 mL | 2.5536 mL | |
| 80 mM | 0.0766 mL | 0.3830 mL | 0.7661 mL | 1.9152 mL | |
| 100 mM | 0.0613 mL | 0.3064 mL | 0.6129 mL | 1.5321 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Bicine Related Classifications
HY-D0868 Related Classifications
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