Transthyretin/TTR Protein, Human (147a.a, HEK293, His)

The transthyretin/TTR protein is a thyroid hormone binder that may be critical for transporting thyroxine from the bloodstream to the brain. Its structure is a homotetramer, forming a dimer of dimers, and its central channel is suggested to play a role in thyroxine binding and transport. Transthyretin/TTR Protein, Human (147a.a, HEK293, His) is the recombinant human-derived Transthyretin/TTR protein, expressed by HEK293 , with C-His labeled tag. The total length of Transthyretin/TTR Protein, Human (147a.a, HEK293, His) is 127 a.a., with molecular weight of ~18.98 kDa.

Transthyretin/TTR Protein serves as a thyroid hormone-binding protein, likely playing a crucial role in transporting thyroxine from the bloodstream to the brain. Forming a homotetramer, it is structured as a dimer of dimers, with subunits assembling around a central channel capable of accommodating two ligand molecules. This suggests a functional significance in the binding and transport of thyroxine. Additionally, Transthyretin/TTR Protein interacts with RBP4, highlighting potential cooperative actions in the regulation of thyroid hormone dynamics. The homotetrameric configuration and ligand-binding properties underscore the importance of Transthyretin/TTR in facilitating the transport and distribution of thyroid hormones, emphasizing its role in maintaining endocrine balance.

1.Measured by its binding ability in a functional ELISA. Immobilized RBP4 at 2.5 μg/mL can bind human TTR. The ED₅₀ for this effect is 960 ng/mL, corresponding to a specific activity is 1.041×10³ Unit/mg.
2.Measured by its binding ability in a functional ELISA.Immobilized recombinant human TTR-His at 10 μg/mL (100 μl/well) can bind recombinant Canine RBP4-Fc with a linear range of 0.3-10.0 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized RBP4 at 2.5 μg/mL can bind human TTR. The ED₅₀ for this effect is 960 ng/mL, corresponding to a specific activity is 1.041×10³ Unit/mg.

Human

HEK293

C-His

P02766 (G21-E147)

7276  [NCBI]

GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE

Approximately 18.98 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4 (Normally trehalose is added as protectant before lyophilization. ) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.