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  3. Fluorescent Dye
  4. HBC514

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1].

For research use only. We do not sell to patients.

HBC514 Chemical Structure

HBC514 Chemical Structure

CAS No. : 2530161-67-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 385 In-stock
Solution
10 mM * 1 mL in DMSO USD 385 In-stock
Solid
1 mg USD 166 In-stock
5 mg USD 350 In-stock
10 mg USD 635 In-stock
25 mg USD 1150 In-stock
50 mg USD 1850 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

HBC514 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1].

IC50 & Target

IC50: RNA fluorescent dye
In Vitro

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells[1].
‘Pepper’ has reduced Mg2+ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness[1].
HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HBC514 Related Antibodies
Molecular Weight

330.43

Formula

C21H22N4
CAS No.
Appearance

Solid

Color

Pink to red

SMILES

CN(CCN(C)C)C(C=C1)=CC=C1/C=C(C#N)/C2=CC=C(C#N)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (75.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0264 mL 15.1318 mL 30.2636 mL
5 mM 0.6053 mL 3.0264 mL 6.0527 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0264 mL 15.1318 mL 30.2636 mL 75.6590 mL
5 mM 0.6053 mL 3.0264 mL 6.0527 mL 15.1318 mL
10 mM 0.3026 mL 1.5132 mL 3.0264 mL 7.5659 mL
15 mM 0.2018 mL 1.0088 mL 2.0176 mL 5.0439 mL
20 mM 0.1513 mL 0.7566 mL 1.5132 mL 3.7829 mL
25 mM 0.1211 mL 0.6053 mL 1.2105 mL 3.0264 mL
30 mM 0.1009 mL 0.5044 mL 1.0088 mL 2.5220 mL
40 mM 0.0757 mL 0.3783 mL 0.7566 mL 1.8915 mL
50 mM 0.0605 mL 0.3026 mL 0.6053 mL 1.5132 mL
60 mM 0.0504 mL 0.2522 mL 0.5044 mL 1.2610 mL
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HBC514 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HBC5142530161-67-0HBC 514HBC-514Fluorescent Dye293T/17HBC fluorophorePepperColor-switchingRNA-imagingSELEXRNA-labelingCOS-7NIH/3T3U-87fluorophore-likedyeGFPInhibitorinhibitorinhibit

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