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  2. HBC514

HBC514 

Cat. No.: HY-133523
Handling Instructions

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells.

For research use only. We do not sell to patients.

HBC514 Chemical Structure

HBC514 Chemical Structure

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Description

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1].

IC50 & Target

IC50: RNA fluorescent dye

In Vitro

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells[1].
‘Pepper’ has reduced Mg2+ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness[1].
HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9)[1].

Molecular Weight

330.43

Formula

C₂₁H₂₂N₄

SMILES

CN(CCN(C)C)C(C=C1)=CC=C1/C=C(C#N)/C2=CC=C(C#N)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

HBC514HBC 514HBC-514293T/17HBC fluorophorePepperColor-switchingRNA-imagingSELEXRNA-labelingCOS-7NIH/3T3U-87fluorophore-likedyeGFPInhibitorinhibitorinhibit

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HBC514
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HY-133523
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