Gel filtration of protected peptides on sephadex G-50 in hexamethylphosphoramide containing 5% water

Gel permeation chromatography on G-50 and G-75 Sephadex gels, using 5% water in hexamethylphosphoramide (phosphoric trisdimethylamide) has been applied to the purification of large protected Peptides which are outside the molecular weight range of the Sephadex LH-20-dimethylformamide system.