2. Academic Validation
  3. Structures of human microsomal cytochrome P450 2A6 complexed with coumarin and methoxsalen

Structures of human microsomal cytochrome P450 2A6 complexed with coumarin and methoxsalen

  • Nat Struct Mol Biol. 2005 Sep;12(9):822-3. doi: 10.1038/nsmb971.
Jason K Yano 1,  Mei-Hui Hsu,  Keith J Griffin,  C David Stout,  Eric F Johnson
Affiliations

Affiliation

  • 1 Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.
PMID: 16086027 DOI: 10.1038/nsmb971
Abstract

Human microsomal Cytochrome P450 2A6 (CYP2A6) contributes extensively to nicotine detoxication but also activates tobacco-specific procarcinogens to mutagenic products. The CYP2A6 structure shows a compact, hydrophobic active site with one hydrogen bond donor, Asn297, that orients coumarin for regioselective oxidation. The inhibitor methoxsalen effectively fills the active site cavity without substantially perturbing the structure. The structure should aid the design of inhibitors to reduce smoking and tobacco-related cancers.

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