2. Academic Validation
  3. Induction of necrotic cell death and mitochondrial permeabilization by heme binding protein 2/SOUL

Induction of necrotic cell death and mitochondrial permeabilization by heme binding protein 2/SOUL

  • FEBS Lett. 2006 Nov 27;580(27):6447-54. doi: 10.1016/j.febslet.2006.10.067.
Andras Szigeti 1,  Szabolcs Bellyei,  Balazs Gasz,  Arpad Boronkai,  Eniko Hocsak,  Orsolya Minik,  Zita Bognar,  Gabor Varbiro,  Balazs Sumegi,  Ferenc Gallyas Jr
Affiliations

Affiliation

  • 1 Institute of Oncotherapy, University of Pécs, Pécs, Hungary.
PMID: 17098234 DOI: 10.1016/j.febslet.2006.10.067
Abstract

We found that heme-binding protein 2/SOUL sensitised NIH3T3 cells to cell death induced by A23187 and etoposide, but it did not affect Reactive Oxygen Species formation. In the presence of sub-threshold calcium, recombinant SOUL provoked mitochondrial permeability transition (mPT) in vitro that was inhibited by cyclosporine A (CsA). This effect was verified in vivo by monitoring the dissipation of mitochondrial membrane potential. Flow cytometry analysis showed that SOUL promoted necrotic death in A23187 and etoposide treated cells, which effect was prevented by CsA. These data suggest that besides its heme-binding properties SOUL promotes necrotic cell death by inducing mPT.

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