Hyperosmotic stress-induced ATF-2 activation through Polo-like kinase 3 in human corneal epithelial cells

Elevated extracellular solute concentration (hyperosmotic stress) perturbs cell function and stimulates cell responses by evoking MAPK cascades and activating AP-1 transcription complex resulting in alterations of gene expression, cell cycle arrest, and Apoptosis. The results presented here demonstrate that hyperosmotic stress elicited increases in ATF-2 phosphorylation through a novel Polo-like kinase 3 (PLK3) pathway in human corneal epithelial (HCE) cells. We found in hyperosmotic stress-induced HCE cells that PLK3 transferred to the nuclear compartment and was colocalized with ATF-2 in nuclei. Kinase activity of PLK3 was significantly activated by hyperosmotic stimulation. Further downstream, active PLK3 phosphorylated ATF-2 at the Thr-71 site in vivo and in vitro. Overexpression of PLK3 and its mutants enhanced hyperosmotic stress-induced ATF-2 phosphorylation. In contrast, suppression of PLK3 by knocking down PLK3 mRNA effectively diminished the effect of hyperosmotic stress-induced ATF-2 phosphorylation. The effect of hyperosmotic stress-induced activation of PLK3 on ATF-2 transcription factor function was also examined in CRE reporter-overexpressed HCE cells. Our results for the first time reveal that hyperosmotic stress can activate the PLK3 signaling pathway that subsequently regulates the AP-1 complex by directly phosphorylating ATF-2 independent from the effects of JNK and p38 activation.