2. Academic Validation
  3. Allelic variation of killer cell immunoglobulin-like receptor 2DS5 impacts glycosylation altering cell surface expression levels

Allelic variation of killer cell immunoglobulin-like receptor 2DS5 impacts glycosylation altering cell surface expression levels

  • Hum Immunol. 2014 Feb;75(2):124-8. doi: 10.1016/j.humimm.2013.11.012.
Noriko K Steiner 1,  Sivanesan Dakshanamurthy 1,  Nicholas Nguyen 1,  Carolyn Katovich Hurley 2
Affiliations

Affiliations

  • 1 Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA.
  • 2 Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA. Electronic address: [email protected].
PMID: 24269691 DOI: 10.1016/j.humimm.2013.11.012
Abstract

Natural killer cell stimulatory receptor gene, KIR2DS5, is polymorphic. While KIR2DS5*002 is most frequently observed, other alleles have also been found. The proteins encoded by these alleles (KIR2DS5*002-*009) are expressed at varying levels on the surface of NKL and Jurkat transfectants. Gel electrophoresis of all allelic products showed two isoforms which differ in the extent of maturation of N-linked glycosylation. These isoforms differed in intensity and molecular weight among the allelic products. Site-directed mutagenesis was used to identify polymorphic variation at residues 123 and 157 as key in altering glycosylation and levels of surface expression.

Keywords

2DL; Cell surface molecules; Human; KIR; Killer cell immunoglobulin-like receptors; Natural killer cells; Polymorphism; killer cell Immunoglobulin-like receptor; two extracellular domains, long cytoplasmic tail.

Figures