2. Academic Validation
  3. ATF5 Connects the Pericentriolar Materials to the Proximal End of the Mother Centriole

ATF5 Connects the Pericentriolar Materials to the Proximal End of the Mother Centriole

  • Cell. 2015 Jul 30;162(3):580-92. doi: 10.1016/j.cell.2015.06.055.
Bhanupriya Madarampalli 1,  Yunsheng Yuan 1,  Dan Liu 1,  Kathleen Lengel 1,  Yidi Xu 1,  Guangfu Li 1,  Jinming Yang 2,  Xinyuan Liu 3,  Zhimin Lu 4,  David X Liu 5
Affiliations

Affiliations

  • 1 Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA.
  • 2 Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA.
  • 3 State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Xinyuan Institute of Medicine and Biotechnology, College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China.
  • 4 Brain Tumor Center and Department of Neuro-Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
  • 5 Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99202, USA. Electronic address: [email protected].
PMID: 26213385 DOI: 10.1016/j.cell.2015.06.055
Abstract

Although it is known that the centrioles play instructive roles in pericentriolar material (PCM) assembly and that the PCM is essential for proper centriole formation, the mechanism that governs centriole-PCM interaction is poorly understood. Here, we show that ATF5 forms a characteristic 9-fold symmetrical ring structure in the inner layer of the PCM outfitting the proximal end of the mother centriole. ATF5 controls the centriole-PCM interaction in a cell-cycle- and centriole-age-dependent manner. Interaction of ATF5 with polyglutamylated tubulin (PGT) on the mother centriole and with PCNT in the PCM renders ATF5 as a required molecule in mother centriole-directed PCM accumulation and in PCM-dependent centriole formation. ATF5 depletion blocks PCM accumulation at the centrosome and causes fragmentation of centrioles, leading to the formation of multi-polar mitotic spindles and genomic instability. These data show that ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the PCM.

Keywords

ATF5; PCM; PCNT; centriole; centrosome; genomic instability; mitotic spindle; polyglutamylated tubulin.

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